Reduction of oxidative stress on DNA and RNA in obese patients after Roux-en-Y gastric bypass surgery-An observational cohort study of changes in urinary markers

被引:13
作者
Carlsson, Elin Rebecka [1 ,2 ]
Fenger, Mogens [1 ]
Henriksen, Trine [3 ]
Kjaer, Laura Kofoed [3 ]
Worm, Dorte [4 ]
Hansen, Dorte Lindqvist [5 ]
Madsbad, Sten [6 ]
Poulsen, Henrik Enghusen [3 ]
机构
[1] Copenhagen Univ Hosp Hvidovre, Dept Clin Biochem, Hvidovre, Denmark
[2] Univ Copenhagen, Nordsjaellands Hosp, Dept Clin Biochem, Hillerod, Denmark
[3] Copenhagen Univ Hosp, Bispebjerg Frederiksberg Hosp, Dept Clin Pharmacol, Copenhagen, Denmark
[4] Amager Hosp, Dept Med, Copenhagen, Denmark
[5] Steno Diabet Ctr Copenhagen, Gentofte, Denmark
[6] Copenhagen Univ Hosp Hvidovre, Dept Endocrinol, Hvidovre, Denmark
关键词
BARIATRIC SURGERY; WEIGHT; DAMAGE; DETERMINANTS; PROFILE; HUMANS; PLASMA; MASS;
D O I
10.1371/journal.pone.0243918
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Increased oxidative stress in obesity and diabetes is associated with morbidity and mortality risks. Levels of oxidative damage to DNA and RNA can be estimated through measurement of 8-oxo-7,8-dihydro-2 ' -deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) in urine. Both markers have been associated with type 2 diabetes, where especially 8-oxoGuo is prognostic for mortality risk. We hypothesized that Roux-en-Y gastric bypass (RYGB) surgery that has considerable effects on bodyweight, hyperglycemia and mortality, might be working through mechanisms that reduce oxidative stress, thereby reducing levels of the urinary markers. We used liquid chromatography coupled with tandem mass spectrometry to analyze the content of 8-oxodG and 8-oxoGuo in urinary samples from 356 obese patients treated with the RYGB-procedure. Mean age (SD) was 44.2 (9.6) years, BMI was 42.1 (5.6) kg/m(2). Ninety-six (27%) of the patients had type 2 diabetes. Excretion levels of each marker before and after surgery were compared as estimates of the total 24-hour excretion, using a model based on glomerular filtration rate (calculated from cystatin C, age, height and weight), plasma- and urinary creatinine. The excretion of 8-oxodG increased in the first months after RYGB. For 8-oxoGuo, a gradual decrease was seen. Two years after RYGB and a mean weight loss of 35 kg, decreased hyperglycemia and insulin resistance, excretion levels of both markers were reduced by approximately 12% (P < 0.001). For both markers, mean excretion levels were about 30% lower in the female subgroup (P < 0.0001). Also, in this subgroup, excretion of 8-oxodG was significantly lower in patients with than without diabetes. We conclude, that oxidative damage to nucleic acids, reflected in the excretion of 8-oxodG and 8-oxoGuo, had decreased significantly two years after RYGB-indicating that reduced oxidative stress could be contributing to the many long-term benefits of RYGB-surgery in obesity and type 2 diabetes.
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页数:19
相关论文
共 49 条
[1]   Weight and Metabolic Outcomes 12 Years after Gastric Bypass [J].
Adams, Ted D. ;
Davidson, Lance E. ;
Litwin, Sheldon E. ;
Kim, Jaewhan ;
Kolotkin, Ronette L. ;
Nanjee, M. Nazeem ;
Gutierrez, Jonathan M. ;
Frogley, Sara J. ;
Ibele, Anna R. ;
Brinton, Eliot A. ;
Hopkins, Paul N. ;
McKinlay, Rodrick ;
Simper, Steven C. ;
Hunt, Steven C. .
NEW ENGLAND JOURNAL OF MEDICINE, 2017, 377 (12) :1143-1155
[2]  
[Anonymous], 2005, BUSINESS CHINA 0228, V31, P5
[3]   Decreased Chromosomal Damage in Lymphocytes of Obese Patients After Bariatric Surgery [J].
Bankoglu, Ezgi Eyluel ;
Arnold, Charlotte ;
Hering, Ilona ;
Hankir, Mohammed ;
Seyfried, Florian ;
Stopper, Helga .
SCIENTIFIC REPORTS, 2018, 8
[4]   Reduction of DNA damage in peripheral lymphocytes of obese patients after bariatric surgery-mediated weight loss [J].
Bankoglu, Ezgi Eyluel ;
Seyfried, Florian ;
Arnold, Charlotte ;
Soliman, Alexander ;
Jurowich, Christian ;
Germer, Christoph Thomas ;
Otto, Christoph ;
Stopper, Helga .
MUTAGENESIS, 2018, 33 (01) :61-67
[5]   Impact of weight loss induced by gastric bypass or caloric restriction on oxidative stress and genomic damage in obese Zucker rats [J].
Bankoglu, Ezgi Eyluel ;
Seyfried, Florian ;
Rotzinger, Laura ;
Nordbeck, Arno ;
Corteville, Caroline ;
Jurowich, Christian ;
Germer, Christoph Thomas ;
Otto, Christoph ;
Stopper, Helga .
FREE RADICAL BIOLOGY AND MEDICINE, 2016, 94 :208-217
[6]   Early Enhancements of Hepatic and Later of Peripheral Insulin Sensitivity Combined With Increased Postprandial Insulin Secretion Contribute to Improved Glycemic Control After Roux-en-Y Gastric Bypass [J].
Bojsen-Moller, Kirstine N. ;
Dirksen, Carsten ;
Jorgensen, Nils B. ;
Jacobsen, Siv H. ;
Serup, Annette K. ;
Albers, Peter H. ;
Hansen, Dorte L. ;
Worm, Dorte ;
Naver, Lars ;
Kristiansen, Viggo B. ;
Wojtaszewski, Jorgen F. P. ;
Kiens, Bente ;
Holst, Jens J. ;
Richter, Erik A. ;
Madsbad, Sten .
DIABETES, 2014, 63 (05) :1725-1737
[7]   Urinary Markers of Nucleic Acid Oxidation and Long-Term Mortality of Newly Diagnosed Type 2 Diabetic Patients [J].
Broedbaek, Kasper ;
Siersma, Volkert ;
Henriksen, Trine ;
Weimann, Allan ;
Petersen, Morten ;
Andersen, Jon T. ;
Jimenez-Solem, Espen ;
Stovgaard, Elisabeth S. ;
Hansen, Lars J. ;
Henriksen, Jan Erik ;
Bonnema, Steen J. ;
Olivarius, Niels de Fine ;
Poulsen, Henrik E. .
DIABETES CARE, 2011, 34 (12) :2594-2596
[8]   Urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine as a biomarker in type 2 diabetes [J].
Broedbaek, Kasper ;
Weimann, Allan ;
Stovgaard, Elisabeth S. ;
Poulsen, Henrik E. .
FREE RADICAL BIOLOGY AND MEDICINE, 2011, 51 (08) :1473-1479
[9]   Reduction in Plasma Levels of Inflammatory and Oxidative Stress Indicators After Roux-En-Y Gastric Bypass [J].
Cabrera, Edilson Joao ;
Valezi, Antonio Carlos ;
Alvares Delfino, Vinicius Daher ;
Lavado, Edson Lopes ;
Barbosa, Decio Sabbatini .
OBESITY SURGERY, 2010, 20 (01) :42-49
[10]   RNA oxidation and iron levels in patients with diabetes [J].
Cejvanovic, Vanja ;
Kjaer, Laura Kofoed ;
Bergholdt, Helle Kirstine Morup ;
Henriksen, Trine ;
Weimann, Allan ;
Ellervik, Christina ;
Poulsen, Henrik Enghusen .
FREE RADICAL BIOLOGY AND MEDICINE, 2018, 129 :532-536