Mapping DNA methylation with high-throughput nanopore sequencing

被引：313
|
作者：
Rand, Arthur C. [1 ,2 ]
Jain, Miten [1 ,2 ]
Eizenga, Jordan M. [1 ,2 ]
Musselman-Brown, Audrey [1 ,2 ]
Olsen, Hugh E. [1 ,2 ]
Akeson, Mark [1 ,2 ]
Paten, Benedict [1 ,2 ]
机构：
[1] Univ Calif Santa Cruz, Dept Biomol Engn, Santa Cruz, CA 95064 USA
[2] Univ Calif Santa Cruz, Genom Inst, Santa Cruz, CA 95064 USA
来源：
NATURE METHODS | 2017年 / 14卷 / 04期
基金：
美国国家卫生研究院;
关键词：
SINGLE-MOLECULE; ESCHERICHIA-COLI; 5-METHYLCYTOSINE; 5-HYDROXYMETHYLCYTOSINE; METHYLOME; ALIGNMENT; ROLES;
D O I：
10.1038/nmeth.4189
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA chemical modifications regulate genomic function. We present a framework for mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.
引用
收藏
页码：411 / +
页数：6
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