共 62 条
Syngeneic Cardiac and Bone Marrow Stromal Cells Display Tissue-Specific microRNA Signatures and microRNA Subsets Restricted to Diverse Differentiation Processes
被引:7
作者:
Meraviglia, Viviana
[1
,2
]
Azzimato, Valerio
[1
,3
]
Piacentini, Luca
[4
]
Chiesa, Mattia
[4
,5
]
Kesharwani, Rupesh K.
[4
,5
]
Frati, Caterina
[6
]
Capogrossi, Maurizio C.
[7
]
Gaetano, Carlo
[8
]
Pompilio, Giulio
[1
,9
]
Colombo, Gualtiero I.
[4
]
Rossini, Alessandra
[9
]
机构:
[1] Ctr Cardiol Monzino IRCCS, Lab Vasc Biol & Regenerat Med, Milan, Italy
[2] Med Univ Lubeck, Affiliated Inst, European Acad Bozen Bolzano EURAC, Ctr Biomed, Bolzano, Italy
[3] Univ Milan, Dept Pharmacol Chemotherapy & Med Toxicol, Milan, Italy
[4] Ctr Cardiol Monzino IRCCS, Lab Immunol & Funct Genom, Milan, Italy
[5] Univ Pavia, Dept Elect Comp & Biomed Engn, I-27100 Pavia, Italy
[6] Univ Parma, Dipartimento Med Interna & Sci Biomed, CISTAC, I-43100 Parma, Italy
[7] Ist Dermopat Immacolata IRCCS, Lab Vasc Pathol, Rome, Italy
[8] Goethe Univ Frankfurt, Dept Cardiol, Div Cardiovasc Epigenet, D-60054 Frankfurt, Germany
[9] Univ Milan, Dept Clin Sci & Community Hlth, Milan, Italy
来源:
PLOS ONE
|
2014年
/
9卷
/
09期
关键词:
MESENCHYMAL STEM-CELLS;
OSTEOGENIC DIFFERENTIATION;
TRANSCRIPTIONAL REGULATION;
IN-VITRO;
EXPRESSION;
ROLES;
MOUSE;
MIRNA;
FIBROBLASTS;
REVEALS;
D O I:
10.1371/journal.pone.0107269
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
MicroRNAs are key modulators at molecular level in different biological processes, including determination of cell fate and differentiation. Herein, microRNA expression profiling experiments were performed on syngeneic cardiac (CStC) and bone marrow (BMStC) mesenchymal stromal cells cultured in standard growth medium and then in vitro exposed to adipogenic, osteogenic, cardiomyogenic and endothelial differentiation media. Analysis identified a tissue-specific microRNA signature composed of 16 microRNAs that univocally discriminated cell type of origin and that were completely unaffected by in vitro differentiation media: 4 microRNAs were over-expressed in cardiac stromal cells, and 12 were overexpressed or present only in bone marrow stromal cells. Further, results revealed microRNA subsets specifically modulated by each differentiation medium, irrespective of the cell type of origin, and a subset of 7 microRNAs that were down-regulated by all media with respect to growth medium. Finally, we identified 16 microRNAs that were differentially modulated by the media when comparing the two tissues of origin. The existence of a tissue-specific microRNA signature surviving to any differentiation stimuli, strongly support the role if microRNAs determining cell identity related to tissue origin. Moreover, we identified microRNA subsets modulated by different culture conditions in a tissue-specific manner, pointing out their importance during differentiation processes.
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页数:16
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