De Novo Sequencing, Assembly, and Annotation of Four Threespine Stickleback Genomes Based on Microfluidic Partitioned DNA Libraries

被引：10

作者：

Berner, Daniel ^{[1
]}

Roesti, Marius ^{[2
,3
,4
]}

Bilobram, Steven ^{[5
]}

Chan, Simon K. ^{[5
]}

Kirk, Heather ^{[5
]}

Pandoh, Pawan ^{[5
]}

Taylor, Gregory A. ^{[5
]}

Zhao, Yongjun ^{[5
]}

Jones, Steven J. M. ^{[5
,6
,7
]}

DeFaveri, Jacquelin ^{[8
]}

机构：

[1] Univ Basel, Dept Environm Sci, Zool, Vesalgasse 1, CH-4051 Basel, Switzerland

[2] Univ British Columbia, Biodivers Res Ctr, Vancouver, BC V6T 1Z4, Canada

[3] Univ British Columbia, Zool Dept, Vancouver, BC V6T 1Z4, Canada

[4] Univ Bern, Inst Ecol & Evolut, Baltzerstr 6, CH-3012 Bern, Switzerland

[5] Canadas Michael Smith Genome Sci Ctr BC Canc, Vancouver, BC V5Z 4S6, Canada

[6] Univ British Columbia, Dept Med Genet, Vancouver, BC V6T 1Z3, Canada

[7] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

[8] Univ Helsinki, Fac Biol & Environm Sci, Viikinkaari 1, FI-00014 Helsinki, Finland

来源：

GENES | 2019年 / 10卷 / 06期

基金：

瑞士国家科学基金会; 芬兰科学院;

关键词：

Gasterosteus aculeatus; genome assembly; STREAM STICKLEBACK; ADAPTIVE DIVERGENCE; PARAPATRIC LAKE; GENE FLOW; EVOLUTION; IMPROVEMENT; SPECIATION;

D O I：

10.3390/genes10060426

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The threespine stickleback is a geographically widespread and ecologically highly diverse fish that has emerged as a powerful model system for evolutionary genomics and developmental biology. Investigations in this species currently rely on a single high-quality reference genome, but would benefit from the availability of additional, independently sequenced and assembled genomes. We present here the assembly of four new stickleback genomes, based on the sequencing of microfluidic partitioned DNA libraries. The base pair lengths of the four genomes reach 92-101% of the standard reference genome length. Together with their de novo gene annotation, these assemblies offer a resource enhancing genomic investigations in stickleback. The genomes and their annotations are available from the Dryad Digital Repository (https://doi.org/10.5061/dryad.113j3h7).

引用

页数：6

共 23 条

[1]

[Anonymous], 2007, Biology of the three-spined stickleback

[2]

[Anonymous], 1994, EVOLUTIONARY BIOL TH

[3] Sexual isolation promotes divergence between parapatric lake and stream stickleback [J].