Regulation of the regenerative activity of dental pulp stem cells from exfoliated deciduous teeth (SHED) of children by TGF-β1 is associated with ALK5/Smad2, TAK1, p38 and MEK/ERK signaling

被引：1
作者：
Chang, Hsiao-Hua [1 ,2 ]
Chen, Il-Ly [1 ,2 ]
Wang, Yin-Lin [1 ,2 ]
Chang, Mei-Chi [3 ,4 ]
Tsai, Yi-Ling [1 ,2 ]
Lan, Wen-Chien [5 ]
Wang, Tong-Mei [1 ,2 ]
Yeung, Sin-Yuet [4 ]
Jeng, Jiiang-Huei [1 ,2 ,6 ,7 ]
机构：
[1] Natl Taiwan Univ, Coll Med, Natl Taiwan Univ Hosp, Dept Dent, Taipei, Taiwan
[2] Natl Taiwan Univ, Coll Med, Sch Dent, Taipei, Taiwan
[3] Chang Gung Univ Sci & Technol, Taoyuan, Taiwan
[4] Chang Gung Mem Hosp, Dept Dent, Taipei, Taiwan
[5] Ching Kuo Inst Management & Hlth, Dept Oral Hyg Care, Keelung, Taiwan
[6] Kaohsiung Med Univ, Sch Dent, Coll Dent Med, Kaohsiung, Taiwan
[7] Kaohsiung Med Univ Hosp, Dept Dent, Kaohsiung, Taiwan
来源：
AGING-US | 2020年 / 12卷 / 21期
关键词：
aging-related diseases; alkaline phosphatase; collagen; cyclooxygenase-2; differentiation; GROWTH-FACTOR-BETA; BONE MORPHOGENETIC PROTEIN-2; TRANSFORMING GROWTH-FACTOR-BETA-1; ALKALINE-PHOSPHATASE; MESENCHYMAL CELLS; APICAL PAPILLA; MESSENGER-RNA; N-CADHERIN; IN-VITRO; EXPRESSION;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Transforming growth factor-beta 1 (TGF-beta 1) regulates wound healing/regeneration and aging processes. Dental pulp stem cells from human exfoliated deciduous teeth (SHED) are cell sources for treatment of age-related disorders. We studied the effect of TGF-beta 1 on SHED and related signaling. SHED were treated with TGF-beta 1 with/without pretreatment/co-incubation by SB431542, U0126, 5Z-7-oxozeaenol or SB203580. Sircol collagen assay, 3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) assay, RT-PCR, western blotting and PathScan phospho-ELISA were used to measure the effects. We found that SHED expressed ALK1, ALK3, ALK5, TGF-RII, betaglycan and endoglin mRNA. TGF-I31 stimulated p-Smad2, p-TAK1, p-ERK, p-p38 and cyclooxygenase-2 (COX-2) protein expression. It enhanced proliferation and collagen content of SHED that were attenuated by SB431542, 5Z-7-oxozeaenol and SB203580, but not U0126. TGF-beta 1 (0.5-1 ng/ml) stimulated ALP of SHED, whereas 5-10 ng/ml TGF-beta 1 suppressed ALP. SB431542 reversed the effects of TGF-beta 1. However, 5Z-7-oxozeaenol, SB203580 and U0126 only reversed the stimulatory effect of TGF beta 1 on ALP. Four inhibitors attenuated TGF-beta 1-induced COX-2 expression. TGF-beta 1-stimulated TIMP-1 and Ncadherin was inhibited by SB431542 and 5Z-7-oxozeaenol. These results indicate that TGF-beta 1 affects SHED by differential regulation of ALK5/Smad2/3, TAK1, p38 and MEK/ERK. TGF-beta 1 and SHED could potentially be used for tissue engineering/regeneration and treatment of age-related diseases.
引用
收藏
页码：21253 / 21272
页数：20
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