Annexin I Stimulates Insulin Secretion through Regulation of Cytoskeleton and PKC Activity

被引:3
作者
Kang, Na-na
Won, Jong Hak [2 ]
Park, Young Min [1 ]
机构
[1] Sungkyunkwan Univ, Dept Biol Sci, Suwon 440746, South Korea
[2] Univ Rochester, Dept Physiol & Pharmacol, Rochester, NY 14642 USA
关键词
Anx I; insulin secretion; cytoskeleton; perifusion; reserve pool; PANCREATIC BETA-CELLS; PROTEIN-KINASE-C; ACTIN-FILAMENTS; LIPOCORTIN; CHROMAFFIN CELLS; GLUCOSE; EXOCYTOSIS; ISLETS; DYNAMICS; MOUSE;
D O I
10.1080/19768354.2009.9647190
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In previous studies, we found that Annexin I (Anx I) was co-secreted with insulin in response to glucose, and that extracellular Anx I stimulated the release of insulin via the Anx I binding site in rat pancreatic islets and the P-cell line. However, the role that Anx I plays in the insulin secretion was not established. Therefore, in this study, we evaluated the insulin secretion pattern in response to Anx I and the involvement of the cytoskeleton or PKC in Anx I-stimulated insulin secretion in MIN6N8a cells. The peak time of insulin secretion in response to Anx I treatment corresponded with the second phase insulin secretion by glucose in the perifused pseudoislets. In addition, Anx I-stimulated insulin secretion was not affect by readily releasable pool depletion. Taken together, these findings indicate that Anx I treatment was associated with movement of the reserve pool of insulin. Furthermore, Anx I-stimulated insulin secretion was attenuated by treatment with a microfilament inhibitor, cytochalasin B, as well as by PKC down regulation. These results indicate that Anx I may be a regulator of second phase insulin secretion.
引用
收藏
页码:17 / 23
页数:7
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