Comparative aspects of energy metabolism in plant trypanosomatids

被引:55
作者
FernandezBecerra, C
SanchezMoreno, M
Osuna, A
Opperdoes, FR
机构
[1] UNIV GRANADA, FAC CIENCIAS, INST BIOTECNOL, GRP BIOQUIM & PARASITOL MOL, E-18071 GRANADA, SPAIN
[2] UNIV CATHOLIQUE LOUVAIN, BIOCHEM UNIT, B-1200 BRUSSELS, BELGIUM
[3] INT INST CELLULAR & MOL PATHOL, TROP DIS RES UNIT, B-1200 BRUSSELS, BELGIUM
关键词
glycolytic enzymes; mitochondrial respiration; H-1 NMR spectroscopy; Phytomonas;
D O I
10.1111/j.1550-7408.1997.tb05734.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The energy metabolism was compared among four different representatives of the genus Phytomonas isolated from different plants and localities: the sieve tubes of the hartrot-infected coconut palm in French Guyana, the latex fluid of Euphorbia hyssopifolia in French Guyana and the fruits of tomato and cherimoya in Spain. All four isolates produced acetate, ethanol, glycerol and glycine as metabolic end-products. In addition, small amounts of succinate and pyruvate were excreted. Only minor quantitative differences were observed in the four isolates. Glycosomes, harboring the glycolytic enzymes, were present in all isolates. No evidence was found for an active involvement of the mitochondrion in metabolism. Respiration was insensitive to the classical inhibitors of the respiratory chain, such as antimycin and potassium cyanide, but inhibited by salicylhydroxamic acid. No evidence was found for the functioning of a citric-acid cycle. It is concluded that representatives of this genus share the same highly active carbohydrate metabolism combined with a complete suppression of mitochondrial activity.
引用
收藏
页码:523 / 529
页数:7
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