Self-assembly of flagellin on Au(111) surfaces

被引：5
作者：
Gonzalez Orive, Alejandro [1 ]
Pissinis, Diego E. [2 ]
Diaz, Carolina [2 ]
Minan, Alejandro [2 ]
Benitez, Guillermo A. [2 ]
Rubert, Aldo [2 ]
Daza Millone, Antonieta [2 ]
Rumbo, Martin [3 ]
Hernandez Creus, Alberto [1 ]
Salvarezza, Roberto C. [2 ]
Schilardi, Patricia L. [2 ]
机构：
[1] Univ La Laguna, Fac Quim, Inst Univ Mat & Nanotecnol IMN, Dept Quim,Unidad Quim Fis, Tenerife 38071, Spain
[2] Univ Nacl La Plata, CONICET, Inst Invest Fisicoquim Teor & Aplicadas INIFTA, Fac Ciencias Exactas,Dept Quim, La Plata, Buenos Aires, Argentina
[3] Univ Nacl La Plata, Fac Ciencias Exactas, Lab Inmunol, La Plata, Buenos Aires, Argentina
来源：
JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2014年 / 433卷
关键词：
Flagellin; Self-assembly; Protein adsorption; Cytochrome C; CYTOCHROME-C; IN-SITU; ELECTRON-TRANSFER; L-CYSTEINE; GOLD; PROTEIN; MONOLAYERS; CONDUCTIVITY; ORIENTATION; ADSORPTION;
D O I：
10.1016/j.jcis.2014.07.016
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The adsorption of flagellin monomers from Pseudomonas fluorescens on Au(111) has been studied by Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), X-ray Photoelectron Spectroscopy (XPS), Surface Plasmon Resonance (SPR), and electrochemical techniques. Results show that flagellin monomers spontaneously self-assemble forming a monolayer thick protein film bounded to the Au surface by the more hydrophobic subunit and exposed to the environment the hydrophilic subunit. The films are conductive and allow allocation of electrochemically active cytochrome C. The self-assembled films could be used as biological platforms to build 3D complex molecular structures on planar metal surfaces and to functionalize metal nanoparticles. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：86 / 93
页数：8
相关论文
共 49 条
  • [11] Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms
    Gorby, Yuri A.
    Yanina, Svetlana
    McLean, Jeffrey S.
    Rosso, Kevin M.
    Moyles, Dianne
    Dohnalkova, Alice
    Beveridge, Terry J.
    Chang, In Seop
    Kim, Byung Hong
    Kim, Kyung Shik
    Culley, David E.
    Reed, Samantha B.
    Romine, Margaret F.
    Saffarini, Daad A.
    Hill, Eric A.
    Shi, Liang
    Elias, Dwayne A.
    Kennedy, David W.
    Pinchuk, Grigoriy
    Watanabe, Kazuya
    Ishii, Shun'ichi
    Logan, Bruce
    Nealson, Kenneth H.
    Fredrickson, Jim K.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (30) : 11358 - 11363
  • [12] Chemical force microscopy study of adhesive properties of polypropylene films: Influence of surface polarity and medium
    Gourianova, S
    Willenbacher, N
    Kutschera, M
    [J]. LANGMUIR, 2005, 21 (12) : 5429 - 5438
  • [13] SCANNING-TUNNELING-MICROSCOPY OF INSULATORS AND BIOLOGICAL SPECIMENS BASED ON LATERAL CONDUCTIVITY OF ULTRATHIN WATER FILMS
    GUCKENBERGER, R
    HEIM, M
    CEVC, G
    KNAPP, HF
    WIEGRABE, W
    HILLEBRAND, A
    [J]. SCIENCE, 1994, 266 (5190) : 1538 - 1540
  • [14] Adsorption and interfacial electron transfer of Saccharomyces cerevisiae yeast cytochrome c monolayers on Au(111) electrodes
    Hansen, AG
    Boisen, A
    Nielsen, JU
    Wackerbarth, H
    Chorkendorff, I
    Andersen, JET
    Zhang, JD
    Ulstrup, J
    [J]. LANGMUIR, 2003, 19 (08) : 3419 - 3427
  • [15] A method for the purification of bacterial flagellin that allows simple upscaling
    Hiriart, Yanina
    Errea, Agustina
    Maciel, Dolores Gonzalez
    Lopez, Juan Carlos
    Rumbo, Martin
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (01) : 15 - 21
  • [16] Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity
    Huleatt, James W.
    Jacobs, Andrea R.
    Tang, Jie
    Desai, Priyanka
    Kopp, Elizabeth B.
    Huang, Yan
    Song, Langzhou
    Nakaar, Valerian
    Powell, T. J.
    [J]. VACCINE, 2007, 25 (04) : 763 - 775
  • [17] TYROSINE PHOSPHATE IN A-TYPE AND B-TYPE FLAGELLINS OF PSEUDOMONAS-AERUGINOSA
    KELLYWINTENBERG, K
    SOUTH, SL
    MONTIE, TC
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (08) : 2458 - 2461
  • [18] A polymerizable GFP variant
    Klein, Agnes
    Toth, Balazs
    Jankovics, Hajnalka
    Muskotal, Adel
    Vonderviszt, Ferenc
    [J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2012, 25 (04) : 153 - 157
  • [19] Optical Anisotropy of Flagellin Layers: In Situ and Label-Free Measurement of Adsorbed Protein Orientation Using OWLS
    Kovacs, Noemi
    Patko, Daniel
    Orgovan, Norbert
    Kurunczi, Sandor
    Ramsden, Jeremy J.
    Vonderviszt, Ferenc
    Horvath, Robert
    [J]. ANALYTICAL CHEMISTRY, 2013, 85 (11) : 5382 - 5389
  • [20] Kozlova Diana, 2014, Virologica Sinica, V29, P33, DOI 10.1007/s12250-014-3379-0
12345