Self-assembly of flagellin on Au(111) surfaces

被引：5

作者：

Gonzalez Orive, Alejandro ^{[1
]}

Pissinis, Diego E. ^{[2
]}

Diaz, Carolina ^{[2
]}

Minan, Alejandro ^{[2
]}

Benitez, Guillermo A. ^{[2
]}

Rubert, Aldo ^{[2
]}

Daza Millone, Antonieta ^{[2
]}

Rumbo, Martin ^{[3
]}

Hernandez Creus, Alberto ^{[1
]}

Salvarezza, Roberto C. ^{[2
]}

Schilardi, Patricia L. ^{[2
]}

机构：

[1] Univ La Laguna, Fac Quim, Inst Univ Mat & Nanotecnol IMN, Dept Quim,Unidad Quim Fis, Tenerife 38071, Spain

[2] Univ Nacl La Plata, CONICET, Inst Invest Fisicoquim Teor & Aplicadas INIFTA, Fac Ciencias Exactas,Dept Quim, La Plata, Buenos Aires, Argentina

[3] Univ Nacl La Plata, Fac Ciencias Exactas, Lab Inmunol, La Plata, Buenos Aires, Argentina

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2014年 / 433卷

关键词：

Flagellin; Self-assembly; Protein adsorption; Cytochrome C; CYTOCHROME-C; IN-SITU; ELECTRON-TRANSFER; L-CYSTEINE; GOLD; PROTEIN; MONOLAYERS; CONDUCTIVITY; ORIENTATION; ADSORPTION;

D O I：

10.1016/j.jcis.2014.07.016

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The adsorption of flagellin monomers from Pseudomonas fluorescens on Au(111) has been studied by Atomic Force Microscopy (AFM), Scanning Tunneling Microscopy (STM), X-ray Photoelectron Spectroscopy (XPS), Surface Plasmon Resonance (SPR), and electrochemical techniques. Results show that flagellin monomers spontaneously self-assemble forming a monolayer thick protein film bounded to the Au surface by the more hydrophobic subunit and exposed to the environment the hydrophilic subunit. The films are conductive and allow allocation of electrochemically active cytochrome C. The self-assembled films could be used as biological platforms to build 3D complex molecular structures on planar metal surfaces and to functionalize metal nanoparticles. (C) 2014 Elsevier Inc. All rights reserved.

引用

收藏

页码：86 / 93

页数：8

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