DNA transport by a micromachined Brownian ratchet device

被引：166

作者：

Bader, JS

Hammond, RW

Henck, SA

Deem, MW

McDermott, GA

Bustillo, JM

Simpson, JW

Mulhern, GT

Rothberg, JM

机构：

[1] CuraGen Corp, New Haven, CT 06511 USA

[2] Univ Calif Berkeley, Dept Elect Engn & Comp Sci, Berkeley, CA 94720 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 23期

关键词：

D O I：

10.1073/pnas.96.23.13165

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have micromachined a silicon-chip device that transports DNA with a Brownian ratchet that rectifies the Brownian motion of microscopic particles. Transport properties for a DNA 50-mer agree with theoretical predictions, and the DNA diffusion constant agrees with previous experiments. This type of micromachine could provide a generic pump or separation component for DNA or other charged species as part of a microscale lab-on-a-chip. A device with reduced feature size could produce a size-based separation of DNA molecules, with applications including the detection of single-nucleotide polymorphisms.

引用

页码：13165 / 13169

页数：5

共 38 条

[1] FLUCTUATION DRIVEN RATCHETS - MOLECULAR MOTORS [J].