Osteoblastic lysosome plays a central role in mineralization

被引：99

作者：

Iwayama, Tomoaki ^{[1
]}

Okada, Tomoko ^{[2
]}

Ueda, Tsugumi ^{[1
]}

Tomita, Kiwako ^{[1
]}

Matsumoto, Shuji ^{[1
]}

Takedachi, Masahide ^{[1
]}

Wakisaka, Satoshi ^{[3
]}

Noda, Takeshi ^{[4
,5
]}

Ogura, Taku ^{[6
]}

Okano, Tomomichi ^{[6
]}

Fratzl, Peter ^{[7
]}

Ogura, Toshihiko ^{[2
]}

Murakami, Shinya ^{[1
]}

机构：

[1] Osaka Univ, Dept Periodontol, Grad Sch Dent, Suita, Osaka 5650871, Japan

[2] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan

[3] Osaka Univ, Dept Oral Anat & Dev, Grad Sch Dent, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Grad Sch Dent, Ctr Frontier Oral Sci, Osaka 5650871, Japan

[5] Osaka Univ, Grad Sch Frontier BioSci, Osaka 5650871, Japan

[6] Lion Corp, Tokyo 1320035, Japan

[7] Max Planck Inst Colloids & Interfaces, Dept Biomat, D-14476 Potsdam, Germany

来源：

SCIENCE ADVANCES | 2019年 / 5卷 / 07期

基金：

日本学术振兴会;

关键词：

AMORPHOUS CALCIUM-PHOSPHATE; MATRIX VESICLES; IN-VITRO; BONE; HYDROXYAPATITE; GROWTH; CELLS; NUCLEATION; CONVERSION; ZEBRAFISH;

D O I：

10.1126/sciadv.aax0672

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mineralization is the most fundamental process in vertebrates. It is predominantly mediated by osteoblasts, which secrete mineral precursors, most likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich and contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs are transported and secreted. Here, we use scanning electron-assisted dielectric microscopy and super-resolution microscopy for assessing live osteoblasts in mineralizing conditions at a nanolevel resolution. We found that the calcium-containing vesicles were multivesicular bodies containing MVs.They were transported via lysosome and secreted by exocytosis. Thus, we present proof that the lysosome transports amorphous calcium phosphate within mineralizing osteoblasts.

引用

页数：9

共 47 条

[1] Impaired calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient mice [J].