Effect of gliclazide on DNA damage in human peripheral blood lymphocytes and insulinoma mouse cells

被引：19
作者：
Sliwinska, A. [1 ]
Blasiak, J.
Drzewoski, J.
机构：
[1] Med Univ Lodz, Dept Internal Med Diabetol & Clin Pharmacol, PL-90131 Lodz, Poland
[2] Univ Lodz, Dept Mol Genet, PL-90131 Lodz, Poland
来源：
CHEMICO-BIOLOGICAL INTERACTIONS | 2006年 / 162卷 / 03期
关键词：
diabetes mellitus type 2; oxidative stress; DNA damage; DNA repair;
D O I：
10.1016/j.cbi.2006.07.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Type 2 diabetes mellitus is associated with increased oxidative stress. Free radicals produced during this stress may damage various cellular components. Gliclazide, a second-generation sulfonylurea, is an oral hypoglycemic drug that possesses antioxidant properties. Therefore, gliclazide may diminish the harmful consequences of oxidative stress in diabetic patients. The aim of our study was to evaluate the action of gliclazide on DNA damage and repair in normal human peripheral blood lymphocytes and insulinoma mouse cells (beta-TC-6). DNA damage and repair were induced by hydrogen peroxide, gamma and ultraviolet radiation and MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) in the presence or absence of gliclazide and were analysed by the alkaline comet assay. DNA double-strand breaks were assayed by pulsed-field gel electrophoresis. Gliclazide protected DNA of both kinds of cells from DNA damage induced by chemicals and radiations. These results suggest that gliclazide may diminish the risk of free radical-related diseases associated with type 2 diabetes mellitus and possibly cancer. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：259 / 267
页数：9
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