Enhancement of soluble protein expression through the use of fusion tags
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作者:
Esposito, Dominic
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NCI, Res Technol Program, Prot Express Lab, SAIC Frederick Inc, Ft Detrick, MD 21702 USANCI, Res Technol Program, Prot Express Lab, SAIC Frederick Inc, Ft Detrick, MD 21702 USA
Esposito, Dominic
[1
]
Chatterjee, Deb K.
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NCI, Res Technol Program, Prot Express Lab, SAIC Frederick Inc, Ft Detrick, MD 21702 USANCI, Res Technol Program, Prot Express Lab, SAIC Frederick Inc, Ft Detrick, MD 21702 USA
Chatterjee, Deb K.
[1
]
机构:
[1] NCI, Res Technol Program, Prot Express Lab, SAIC Frederick Inc, Ft Detrick, MD 21702 USA
The soluble expression of heterologous proteins in Escherichia coli remains a serious bottleneck in protein production. Although alteration of expression conditions can sometimes solve the problem, the best available tools to date have been fusion tags that enhance the solubility of expressed proteins. However, a systematic analysis of the utility of these solubility fusions has been difficult, and it appears that many proteins react differently to the presence of different solubility tags. The advent of high-throughput structural genomics programs and advances in cloning and expression technology afford us a new way to compare the effectiveness of solubility tags. This data should allow us to better predict the effectiveness of tags currently in use, and might also provide the information needed to identify new fusion tags.
机构:
Univ London Imperial Coll Sci & Technol, Biol Struct & Funct Sect, Div Biomed Sci, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci & Technol, Biol Struct & Funct Sect, Div Biomed Sci, Fac Med, London SW7 2AZ, England
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Monash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, AustraliaMonash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, Australia
Daly, R
;
Hearn, MTW
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Monash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, AustraliaMonash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, Australia
机构:
Univ London Imperial Coll Sci & Technol, Biol Struct & Funct Sect, Div Biomed Sci, Fac Med, London SW7 2AZ, EnglandUniv London Imperial Coll Sci & Technol, Biol Struct & Funct Sect, Div Biomed Sci, Fac Med, London SW7 2AZ, England
机构:
Monash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, AustraliaMonash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, Australia
Daly, R
;
Hearn, MTW
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机构:
Monash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, AustraliaMonash Univ, ARC, Special Res Ctr Green Chem, Clayton, Vic 3800, Australia