Effect of ionizing radiation after-therapy interval on bone: histomorphometric and biomechanical characteristics

被引：17

作者：

Ferreira Soares, Priscilla Barbosa ^{[1
]}

Soares, Carlos Jose ^{[2
]}

Justino Oliveira Limirio, Pedro Henrique ^{[1
]}

Rezende de Jesus, Rainde Naiara ^{[3
]}

Dechichi, Paula ^{[4
]}

Spin-Neto, Rubens ^{[5
]}

Zanetta-Barbosa, Darceny ^{[3
]}

机构：

[1] Univ Fed Uberlandia, Sch Dent, Dept Periodontol & Implantol, Ave Para S-N,Campus Umuarama,Bloco 4L, BR-38400902 Uberlandia, MG, Brazil

[2] Univ Fed Uberlandia, Sch Dent, Dept Operat Dent & Dent Mat, Ave Para S-N,Campus Umuarama,Bloco 4L, BR-38400902 Uberlandia, MG, Brazil

[3] Univ Fed Uberlandia, Sch Dent, Dept Oral & Maxillofacial Surg, Ave Para S-N,Campus Umuarama,Bloco 4L, BR-38400902 Uberlandia, MG, Brazil

[4] Univ Fed Uberlandia, Inst Biomed Sci, Ave Para 1720,Campus Umuarama,Bloco 2B, BR-38400902 Uberlandia, MG, Brazil

[5] Aarhus Univ, Dept Dent & Oral Hlth, Dept Oral Radiol, Vennelyst Blvd 9,Bldg 1613,130, DK-8000 Aarhus C, Denmark

来源：

CLINICAL ORAL INVESTIGATIONS | 2019年 / 23卷 / 06期

关键词：

Ionizing radiation; Bone; Biomechanics; Microcomputed tomography; X-RAY-IRRADIATION; CORTICAL BONE; DENTAL IMPLANTS; TISSUE; OSTEORADIONECROSIS; NANOINDENTATION; MINERALIZATION; RADIOTHERAPY; STRENGTH; CREEP;

D O I：

10.1007/s00784-018-2724-3

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

ObjectivesThis study aimed to evaluate the effects of radiotherapy on biomechanical, histomorphometric, and microstructural characteristics of bone, in diverse periods, compared with intact bone tissue.Materials and methodsEighteen adult male New Zealand rabbits were treated with a single radiation dose of 30Gy. The animals were randomly divided into six groups: NoIr, control group, no radiation, and five irradiated groups sacrificed after 24h (Ir24h), 7 (Ir7d), 14 (Ir14d), 21 (Ir21d), and 28 (Ir28d) days. After these periods, the animals were sacrificed and their tibias (n=6) evaluated using three-point bending test to calculate the ultimate force, work to failure, and bone stiffness. Dynamic indentation test was used to quantify Vickers hardness and elasticity modulus of bone tissue. Micro-CT was used to analyze the cortical volume (CtV), cortical thickness (CtTh), and porosity (Ct.Po). Histomorphometric assessment was based on the lacunarity of bone tissue. Data were analyzed using one-way ANOVA and Kruskal-Wallis tests followed by Tukey, Dunnet, and Dunn's post-tests (P<0.05).ResultsThe ultimate force, work to failure, stiffness, elastic modulus, and Vickers hardness values of irradiated bone were significantly lower that non-irradiated bone. Irradiated bone showed significantly lower CtTh and CtV values and higher CtPo than non-irradiated bone. No significant difference was found for lacunarity between non-irradiated bone and irradiated bone.ConclusionsIonizing radiation decreases normal anisotropy on microarchitecture of cortical bone, and increases bone fragility compared with non-irradiated bone. Further, these changes were seen after longer periods (e.g., 14 and 21days), and not immediately after radiation therapy.Clinical relevanceThe radiotherapy reduces bone mechanical properties and the normal structure of organic and inorganic bone matrix. For studying the protocols to protect the radiotherapy effect using rabbit model, the use of the sacrificing period between 14 and 21days is recommended.

引用

页码：2785 / 2793

页数：9

共 37 条

[1] The Influence of Radiation Therapy on Dental Implantology [J].