Development of a solid-phase extraction procedure for the simultaneous determination of polyphenols, organic acids and sugars in wine

被引：58

作者：

de Villiers, A ^{[1
]}

Lynen, F ^{[1
]}

Crouch, A ^{[1
]}

Sandra, P ^{[1
]}

机构：

[1] Univ Stellenbosch, CENSSUS, Dept Chem, ZA-7602 Matieland, South Africa

来源：

CHROMATOGRAPHIA | 2004年 / 59卷 / 7-8期

关键词：

column liquid chromatography; evaporative light scattering and UV detection; solid-phase extraction; polyphenols; sugars and organic acids; wine;

D O I：

10.1365/s10337-0040204-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A solid-phase extraction (SPE) procedure that fractionates wine samples into 2 sub-samples containing sugars and organic acids (sub-sample 1) and low molecular weight polyphenols (sub-sample 2), respectively, together with rugged LC procedures for their analyses are described. Wine is adjusted to pH 2.5 and loaded on a styrene-divinylbenzene (SDB) cartridge. The organic acids and sugars are eluted with 20 mM sulphuric acid and the monomeric polyphenols with ethyl acetate. Glucose and fructose are analysed by normal phase LC with evaporative light scattering detection and the organic acids by ion exclusion chromatography with UV detection at 210 nm. Analysis of the phenolic fraction is performed by reversed phase LC with diode array detection. Recoveries and repeatability's for 27 standard compounds (2 sugars, 7 organic acids and 18 polyphenols) are presented. The method represents an improvement in terms of productivity and robustness compared to currently used procedures.

引用

页码：403 / 409

页数：7

共 38 条

[1] Method development and validation for the simultaneous determination of organic and inorganic acids by capillary zone electrophoresis [J].