Expanding application of CRISPR-Cas9 system in microorganisms

被引：36
作者：
Zhao, Jing [1 ,2 ]
Fang, Huan [1 ,2 ]
Zhang, Dawei [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
[2] Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY | 2020年 / 5卷 / 04期
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
CRISPR-Cas9; system; Microorganisms; Off-target; Cas9; toxicity; sgRNA; SEQUENCE-SPECIFIC CONTROL; ESCHERICHIA-COLI; RNA; GENE; EXPRESSION; CAS9; SPECIFICITY; INTEGRATION; ACTIVATION; BACTERIA;
D O I：
10.1016/j.synbio.2020.08.001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering, synthetic biology, and systems biology. The CRISPR-Cas9 and its derivative tools are one of the best choices for precise genome editing, multiplexed genome editing, and reversible gene expression control in microorganisms. However, challenges remain for applying CRISPR-Cas9 in novel microorganisms, especially those industrial microorganism hosts that are intractable using traditional genetic manipulation tools. How to further extend CRISPR-Cas9 to these microorganisms is being an urgent matter. In this review, we first introduce the mechanism and application of CRISPR-Cas9, then discuss how to optimize CRISPR-Cas9 as genome editing tools, including but not limited to how to reduce off-target effects and Cas9 related toxicity, and how to increase on-target efficiency by optimizing crRNA and sgRNA design.
引用
收藏
页码：269 / 276
页数：8
相关论文
共 82 条
[1]  
[Anonymous], 2013, CELL
[2]   CRISPR provides acquired resistance against viruses in prokaryotes [J].
Barrangou, Rodolphe ;
Fremaux, Christophe ;
Deveau, Helene ;
Richards, Melissa ;
Boyaval, Patrick ;
Moineau, Sylvain ;
Romero, Dennis A. ;
Horvath, Philippe .
SCIENCE, 2007, 315 (5819) :1709-1712
[3]   Rapid and Efficient One-Step Metabolic Pathway Integration in E-coli [J].
Bassalo, Marcelo C. ;
Garst, Andrew D. ;
Halweg-Edwards, Andrea L. ;
Grau, William C. ;
Domaille, Dylan W. ;
Mutalik, Vivek K. ;
Arkin, Adam P. ;
Gill, Ryan T. .
ACS SYNTHETIC BIOLOGY, 2016, 5 (07) :561-568
[4]   Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system [J].
Bikard, David ;
Jiang, Wenyan ;
Samai, Poulami ;
Hochschild, Ann ;
Zhang, Feng ;
Marraffini, Luciano A. .
NUCLEIC ACIDS RESEARCH, 2013, 41 (15) :7429-7437
[5]   Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin [J].
Bolotin, A ;
Ouinquis, B ;
Sorokin, A ;
Ehrlich, SD .
MICROBIOLOGY-SGM, 2005, 151 :2551-2561
[6]   DNA-binding-domain fusions enhance the targeting range and precision of Cas9 [J].
Bolukbasi, Mehmet Fatih ;
Gupta, Ankit ;
Oikemus, Sarah ;
Derr, Alan G. ;
Garber, Manuel ;
Brodsky, Michael H. ;
Zhu, Lihua Julie ;
Wolfe, Scot A. .
NATURE METHODS, 2015, 12 (12) :1150-+
[7]  
Burgess D, 2013, NAT REV GENET, V14
[8]  
Chapman JE., 2017, Appl Biosaf, DOI [10.1177/1535676017694148, DOI 10.1177/1535676017694148]
[9]   BIOTECHNOLOGY Rewriting a genome [J].
Charpentier, Emmanuelle ;
Doudna, Jennifer A. .
NATURE, 2013, 495 (7439) :50-51
[10]   Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System [J].
Chen, Baohui ;
Gilbert, Luke A. ;
Cimini, Beth A. ;
Schnitzbauer, Joerg ;
Zhang, Wei ;
Li, Gene-Wei ;
Park, Jason ;
Blackburn, Elizabeth H. ;
Weissman, Jonathan S. ;
Qi, Lei S. ;
Huang, Bo .
CELL, 2013, 155 (07) :1479-1491
123456789