Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells

被引：11

作者：

Sun, Yun-Peng ^{[1
]}

Zhang, Ben-Long ^{[2
]}

Duan, Jian-Wen ^{[1
]}

Wu, Huan-Huan ^{[3
]}

Wang, Ben-Quan ^{[1
]}

Yu, Zheng-Ping ^{[1
]}

Yang, Wen-Jun ^{[1
]}

Shan, Yun-Feng ^{[1
]}

Zhou, Meng-Tao ^{[1
]}

Zhang, Qi-Yu ^{[1
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Wenzhou 325015, Zhejiang, Peoples R China

[2] Yiwu Chouzhou Hosp, Dept Gen Surg, Yiwu 322000, Zhejiang, Peoples R China

[3] Wenzhou Med Univ, Affiliated Hosp 1, Dept Infect Dis, Wenzhou 325015, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2014年 / 15卷 / 03期

关键词：

NK4; pancreatic cancer; bone marrow-derived mesenchymal stem cells; transduction; HEPATOCYTE GROWTH-FACTOR; TUMOR-GROWTH; C-MET; TARGETED-DELIVERY; INHIBITION; RECEPTOR; ANGIOGENESIS; THERAPY; GENE; MIGRATION;

D O I：

10.3390/ijms15033729

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer.

引用

页码：3729 / 3745

页数：17

共 46 条

[1] Pancreatic cancer biology and genetics [J].

Bardeesy, N ;

DePinho, RA .

NATURE REVIEWS CANCER, 2002, 2 (12) :897-909

[2]

BELLUSCI S, 1994, ONCOGENE, V9, P1091

[3] Isolation and Hepatocyte Differentiation of Mesenchymal Stem Cells From Porcine Bone Marrow-"Surgical Waste" as a Novel MSC Source [J].

Brueckner, S. ;

Tautenhahn, H. -M. ;

Winkler, S. ;

Stock, P. ;

Jonas, S. ;

Dollinger, M. ;

Christ, B. .

TRANSPLANTATION PROCEEDINGS, 2013, 45 (05) :2056-2058

[4] CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment [J].

Burger, JA ;

Kipps, TJ .

BLOOD, 2006, 107 (05) :1761-1767

[5] Hepatocyte growth factor is a lymphanoiogenic factor with an indirect mechanism of action [J].

Cao, RH ;

Björndahl, MA ;

Gallego, MI ;

Chen, SH ;

Religa, P ;

Hansen, AJ ;

Cao, YH .

BLOOD, 2006, 107 (09) :3531-3536

[6] Mesenchymal stem cells as trophic mediators [J].

Caplan, Arnold I. ;

Dennis, James E. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 98 (05) :1076-1084

[7] Diosgenin Suppresses Hepatocyte Growth Factor (HGF)-Induced Epithelial-Mesenchymal Transition by Down-regulation of Mdm2 and Vimentin [J].

Chang, Hsiang-Yu ;

Kao, Ming-Ching ;

Way, Tzong-Der ;

Ho, Chi-Tang ;

Fu, Earl .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (10) :5357-5363

[8] Akt serine threonine kinase regulates platelet-derived growth factor-induced DNA synthesis in glomerular mesangial cells -: Regulation of c-fos and p27kip1 gene expression [J].

Choudhury, GG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (38) :35636-35643

[9] Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells [J].

Chow, Kelsey S. ;

Jun, DuHyun ;

Helm, Karen M. ;

Wagner, David H. ;

Majka, Susan M. .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2011, (56)

[10] Inhibition of tumor growth and invasion by a four-kringle antagonist (HGF/NK4) for hepatocyte growth factor [J].

Date, K ;

Matsumoto, K ;

Kuba, K ;

Shimura, H ;

Tanaka, M ;

Nakamura, T .

ONCOGENE, 1998, 17 (23) :3045-3054

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