Study on interactions of several psychopharmaceutical drugs with β-cycloclextrin by affinity capillary electrophoresis and their application in separation

The binding of psychopharmaceutical drugs by beta-cyclodextrin (beta-CD) changed their effective electrophoretic mobilities. Based on this phenomenon the interactions between some psychopharmaceutical drugs and beta-cyclodextrin were studied and the binding constants of beta-CD with tiapride, imipromine, clomipramine, amitriptyline, trifluoperazine, perphenazine and carbamazepine were determined by affinity capillary electrophoresis. The presence of beta-CD improved the separation of these psychopharmaceutical drugs. Organic modifier was used as another kind of additive to be added in the running buffer to improve further the separation. 70 mM pH 7.2 phosphate buffer solution containing 12 mM beta-cyclodextrin and 15% (v/v) acetone was selected as optimal running buffer for separation by capillary electrophoresis.