In vitro binding of nucleolin to double-stranded telomeric DNA

被引：18

作者：

Pollice, A

Zibella, MP

Bilaud, T

Laroche, T

Pulitzer, JF

Gilson, E

机构：

[1] Univ Naples Federico II, Dipartimento Genet Biol Gen & Mol, I-80134 Naples, Italy

[2] Int Inst Genet & Biophys, I-80100 Naples, Italy

[3] Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland

[4] Ecole Normale Super Lyon, CNRS, Biol Cellulaire & Mol Lab, UMR5665, F-69364 Lyon 07, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 268卷 / 03期

关键词：

telomere; nucleolin; DNA-binding; mammalian;

D O I：

10.1006/bbrc.2000.2237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus. (C) 2000 Academic Press.

引用

页码：909 / 915

页数：7

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