Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection

被引：8

作者：

El-Ashram, Saeed ^{[1
,2
,3
,5
]}

Yin, Qing ^{[1
,2
,3
]}

Barta, John R. ^{[6
]}

Khan, Jamal ^{[1
,2
,3
]}

Liu, Xianyong ^{[1
,2
,3
]}

Suo, Xun ^{[1
,2
,3
,4
]}

机构：

[1] China Agr Univ, Natl Anim Protozoa Lab, Beijing 100193, Peoples R China

[2] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

[3] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

[4] Minist Agr, Key Lab Anim Epidemiol & Zoonosis, Beijing 100193, Peoples R China

[5] Kafr El Sheikh Univ, Fac Sci, Kafr Al Sheikh, Egypt

[6] Univ Guelph, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 119卷

基金：

中国国家自然科学基金;

关键词：

Immunoproteomic technology; Diagnosis; Toxoplasmosis; DIFFERENCE-GEL-ELECTROPHORESIS; RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; ANTIGENIC PROTEINS; PROTEOMIC ANALYSIS; MASS-SPECTROMETRY; IDENTIFICATION; EIMERIA; SERODIAGNOSIS; IMMUNOBLOT; EXPRESSION;

D O I：

10.1016/j.mimet.2015.09.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoproteomic technology offers an exceptional tool to fill the blanks that still exist in diagnosis of Toxoplasma gondii infection despite its annotated sequence. The pitfalls of serological assays and current immunoproteomic approaches are accentuated, and new approaches are presented to improve the signal and to eliminate the noise produced by blocking-specific background. This review also highlights examples where immunoproteomic studies have contributed to broaden our understanding of toxoplasmosis diagnosis. Further promising solutions, which immunoproteomic technology can grant for toxoplasmosis diagnosis are part of an intense discussion. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：18 / 30

页数：13

共 85 条

[1] Strain and stage specific variation in Toxoplasma gondii antigens [J].