Can the detection dog alert on COVID-19 positive persons by sniffing axillary sweat samples? A proof-of-concept study

被引：60

作者：

Grandjean, Dominique ^{[1
]}

Sarkis, Riad ^{[2
]}

Lecoq-Julien, Clothilde ^{[1
]}

Benard, Aymeric ^{[3
]}

Roger, Vinciane ^{[1
]}

Levesque, Eric ^{[4
]}

Bernes-Luciani, Eric ^{[3
]}

Maestracci, Bruno ^{[3
]}

Morvan, Pascal ^{[5
]}

Gully, Eric ^{[6
]}

Berceau-Falancourt, David ^{[7
]}

Haufstater, Pierre ^{[8
]}

Herin, Gregory ^{[9
]}

Cabrera, Joaquin ^{[1
]}

Muzzin, Quentin ^{[1
]}

Gallet, Capucine ^{[1
]}

Bacque, Helene ^{[1
]}

Broc, Jean-Marie ^{[10
]}

Thomas, Leo ^{[10
]}

Lichaa, Anthony ^{[2
]}

Moujaes, Georges ^{[2
]}

Saliba, Michele ^{[2
]}

Kuhn, Aurore ^{[10
]}

Galey, Mathilde ^{[10
]}

Berthail, Benoit ^{[10
]}

Lapeyre, Lucien ^{[4
]}

Capelli, Anthoni ^{[5
]}

Renault, Steevens ^{[5
]}

Bachir, Karim ^{[5
]}

Kovinger, Anthony ^{[5
]}

Comas, Eric ^{[6
]}

Stainmesse, Aymeric ^{[6
]}

Etienne, Erwan ^{[6
]}

Voeltzel, Sebastien ^{[6
]}

Mansouri, Sofiane ^{[6
]}

Berceau-Falancourt, Marlene ^{[11
]}

Dami, Aime ^{[3
]}

Charlet, Lary ^{[6
]}

Ruau, Eric ^{[6
]}

Issa, Mario ^{[2
]}

Grenet, Carine ^{[12
]}

Billy, Christophe ^{[13
]}

Tourtier, Jean-Pierre ^{[4
]}

Desquilbet, Loic ^{[1
]}

机构：

[1] Univ Paris Est, Ecole Natl Vet Alfort, Maisons Alfort, France

[2] Univ Franco Libanaise St Joseph, Fac Med, Beirout, Lebanon

[3] Serv Incendie & Secours Corse Sud, Ajaccio, France

[4] Univ Paris Est Creteil, Ctr Hosp Univ Henri Mondor, Maisons Alfort, France

[5] DiagNose, Neuvy, France

[6] Serv Dept Incendie & Secours Seine & Marne, Melun, France

[7] Compagnie Cynophile Prefecture Police, Paris, France

[8] Hop Univ Pitie Salpetriere, Paris, France

[9] Biodes SAS, Strasbourg, France

[10] Hop Instruct Armees Begin, St Mande, France

[11] Cynopro Dectect Dogs, Paris, France

[12] Grand Hop Est Francilien, Jossigny, France

[13] Ctr Hosp Francois Quesnay, Mantes La Jolie, France

来源：

PLOS ONE | 2020年 / 15卷 / 12期

关键词：

CANINE OLFACTORY DETECTION; PROSTATE-CANCER; SCENT-DETECTION; BLADDER-CANCER; LUNG; IDENTIFICATION; DIAGNOSIS; IDENTIFY; URINE; SPECTROMETRY;

D O I：

10.1371/journal.pone.0243122

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this proof-of-concept study was to evaluate if trained dogs could discriminate between sweat samples from symptomatic COVID-19 positive individuals (SARS-CoV-2 PCR positive) and those from asymptomatic COVID-19 negative individuals. The study was conducted at 2 sites (Paris, France, and Beirut, Lebanon), followed the same training and testing protocols, and involved six detection dogs (three explosive detection dogs, one search and rescue dog, and two colon cancer detection dogs). A total of 177 individuals were recruited for the study (95 symptomatic COVID-19 positive and 82 asymptomatic COVID-19 negative individuals) from five hospitals, and one underarm sweat sample per individual was collected. The dog training sessions lasted between one and three weeks. Once trained, the dog had to mark the COVID-19 positive sample randomly placed behind one of three or four olfactory cones (the other cones contained at least one COVID-19 negative sample and between zero and two mocks). During the testing session, a COVID-19 positive sample could be used up to a maximum of three times for one dog. The dog and its handler were both blinded to the COVID-positive sample location. The success rate per dog (i.e., the number of correct indications divided by the number of trials) ranged from 76% to 100%. The lower bound of the 95% confidence interval of the estimated success rate was most of the time higher than the success rate obtained by chance after removing the number of mocks from calculations. These results provide some evidence that detection dogs may be able to discriminate between sweat samples from symptomatic COVID-19 individuals and those from asymptomatic COVID-19 negative individuals. However, due to the limitations of this proof-of-concept study (including using some COVID-19 samples more than once and potential confounding biases), these results must be confirmed in validation studies.

引用

页数：19