Analysis on Blast Fungus-Responsive Characters of a Flavonoid Phytoalexin Sakuranetin; Accumulation in Infected Rice Leaves, Antifungal Activity and Detoxification by Fungus

被引:74
作者
Hasegawa, Morifumi [1 ]
Mitsuhara, Ichiro [2 ]
Seo, Shigemi [2 ]
Okada, Kazunori [3 ]
Yamane, Hisakazu [4 ]
Iwai, Takayoshi [5 ]
Ohashi, Yuko [2 ]
机构
[1] Ibaraki Univ, Coll Agr, Ami, Ibaraki 3000393, Japan
[2] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
[3] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
[4] Teikyo Univ, Dept Biosci, Utsunomiya, Tochigi 3208551, Japan
[5] Miyagi Univ, Sch Food Agr & Environm Sci, Taihaku Ku, Sendai, Miyagi 9820215, Japan
关键词
antifungal activity; blast fungus; detoxification; flavonoid; hypersensitive reaction (HR); momilactone A; phytoalexin (PA); resistance; rice; sakuranetin; DISEASE RESISTANCE; ETHYLENE BIOSYNTHESIS; MAGNAPORTHE-GRISEA; PLANTS; IDENTIFICATION; DIAZOMETHANE; METABOLISM;
D O I
10.3390/molecules190811404
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To understand the role of the rice flavonoid phytoalexin (PA) sakuranetin for blast resistance, the fungus-responsive characteristics were studied. Young rice leaves in a resistant line exhibited hypersensitive reaction (HR) within 3 days post inoculation (dpi) of a spore suspension, and an increase in sakuranetin was detected at 3 dpi, increasing to 4-fold at 4 dpi. In the susceptible line, increased sakuranetin was detected at 4 dpi, but not at 3 dpi, by which a large fungus mass has accumulated without HR. Induced expression of a PA biosynthesis gene OsNOMT for naringenin 7-O-methyltransferase was found before accumulation of sakuranetin in both cultivars. The antifungal activity of sakuranetin was considerably higher than that of the major rice diterpenoid PA momilactone A in vitro and in vivo under similar experimental conditions. The decrease and detoxification of sakuranetin were detected in both solid and liquid mycelium cultures, and they took place slower than those of momilactone A. Estimated local concentration of sakuranetin at HR lesions was thought to be effective for fungus restriction, while that at enlarged lesions in susceptible rice was insufficient. These results indicate possible involvement of sakuranetin in blast resistance and its specific relation to blast fungus.
引用
收藏
页码:11404 / 11418
页数:15
相关论文
共 25 条
[1]   Synthesis of 7-methoxyapigeninidin and its fungicidal activity against Gloeocercospora sorghi [J].
Aida, Y ;
Tamogami, S ;
Kodama, O ;
Tsukiboshi, T .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (09) :1495-1496
[2]   Differences in phytoalexin response among rice cultivars of different resistance to blast [J].
Dillon, VM ;
Overton, J ;
Grayer, RJ ;
Harborne, JB .
PHYTOCHEMISTRY, 1997, 44 (04) :599-603
[3]   SIMPLE DEVICE FOR PREPARING ETHEREAL DIAZOMETHANE WITHOUT RESORTING TO CODISTILLATION [J].
FALES, HM ;
JAOUNI, TM ;
BABASHAK, JF .
ANALYTICAL CHEMISTRY, 1973, 45 (13) :2302-2303
[4]   Plant-fungal interactions: the search for phytoalexins and other antifungal compounds from higher plants [J].
Grayer, RJ ;
Kokubun, T .
PHYTOCHEMISTRY, 2001, 56 (03) :253-263
[5]   Phytoalexin transgenics in crop protection-Fairy tale with a happy end? [J].
Grosskinsky, Dominik K. ;
van der Graaff, Eric ;
Roitsch, Thomas .
PLANT SCIENCE, 2012, 195 :54-70
[6]   Phytoalexin Accumulation in the Interaction Between Rice and the Blast Fungus [J].
Hasegawa, Morifumi ;
Mitsuhara, Ichiro ;
Seo, Shigemi ;
Imai, Takuya ;
Koga, Jinichiro ;
Okada, Kazunori ;
Yamane, Hisakazu ;
Ohashi, Yuko .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2010, 23 (08) :1000-1011
[7]   Identification of a Degradation Intermediate of the Momilactone A Rice Phytoalexin by the Rice Blast Fungus [J].
Imai, Takuya ;
Ohashi, Yuko ;
Mitsuhara, Ichiro ;
Seo, Shigemi ;
Toshima, Hiroaki ;
Hasegawa, Morifumi .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2012, 76 (02) :414-416
[8]   Identification of a Novel Casbane-Type Diterpene Phytoalexin, ent-10-Oxodepressin, from Rice Leaves [J].
Inoue, Yasuno ;
Sakai, Miki ;
Yao, Qun ;
Tanimoto, Yosuke ;
Toshima, Hiroaki ;
Hasegawa, Morifumi .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2013, 77 (04) :760-765
[9]  
ISE K, 1988, BREED SCI S2, V38, P404
[10]   Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants [J].
Iwai, Takayoshi ;
Miyasaka, Atsushi ;
Seo, Shigemi ;
Ohashi, Yuko .
PLANT PHYSIOLOGY, 2006, 142 (03) :1202-1215