Multifunctional thin films of lactoferrin for biochemical use deposited by MAPLE technique

被引：28

作者：

Constantinescu, Catalin ^{[1
]}

Palla-Papavlu, Alexandra ^{[1
]}

Rotaru, Andrei ^{[1
]}

Florian, Paula ^{[2
]}

Chelu, Florica ^{[2
]}

Icriverzi, Madalina ^{[2
]}

Nedelcea, Anca ^{[1
]}

Dinca, Valentina ^{[1
]}

Roseanu, Anca ^{[2
]}

Dinescu, Maria ^{[1
]}

机构：

[1] INFLPR, Natl Inst Laser Plasma & Radiat Phys, RO-077125 Bucharest, Romania

[2] Acad Romana, Inst Biochem, RO-060031 Bucharest, Romania

来源：

APPLIED SURFACE SCIENCE | 2009年 / 255卷 / 10期

关键词：

MAPLE; Lactoferrin; Thin films; Proteins; PULSED-LASER EVAPORATION; BOVINE SERUM-ALBUMIN; SURFACE-MORPHOLOGY; BACTERIAL BIOFILMS; MATRIX; POLYMER; GROWTH;

D O I：

10.1016/j.apsusc.2008.07.163

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Lactoferrin (Lf) is an iron-binding glycoprotein present in almost all mammalian secretions which plays an important role in host defense against microbial and viral infections. The protein has been reported to also have anti-inflammatory activity and antitumoral effects in vitro and in vivo. Thin films of Lf were deposited on silicon, quartz and Thermanox plastic coverslip substrates by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique, using a Nd:YAG laser working at 266 nm, at different laser fluences (0.1-0.8 J cm(2)). The deposited layers have been characterized by Fourier Transformed Infra-Red spectroscopy (FTIR), and the morphology of the various substrates was investigated by Atomic Force Microscopy (AFM). The biocompatibility of lactoferrin thin films was evaluated for each substrate, by in vitro biochemical tests. (C) 2008 Elsevier B. V. All rights reserved.

引用

页码：5491 / 5495

页数：5

共 35 条

[1]

[Anonymous], 2007, PULSED LASER DEPOSIT, DOI DOI 10.1111/adj.12093

[2] The effect of the matrix on film properties in matrix-assisted pulsed laser evaporation [J].

Bubb, DM ;

Wu, PK ;

Horwitz, JS ;

Callahan, JH ;

Galicia, M ;

Vertes, A ;

McGill, RA ;

Houser, EJ ;

Ringeisen, BR ;

Chrisey, DB .

JOURNAL OF APPLIED PHYSICS, 2002, 91 (04) :2055-2058

[3]

CALIFANO V, 2008, APPL SURF SCI ONLINE

[4] Laser deposition of polymer and biomaterial films [J].

Chrisey, DB ;

Piqué, A ;

McGill, RA ;

Horwitz, JS ;

Ringeisen, BR ;

Bubb, DM ;

Wu, PK .

CHEMICAL REVIEWS, 2003, 103 (02) :553-576

[5] Thin films of polyaniline deposited by MAPLE technique [J].

Constantinescu, C. ;

Scarisoreanu, N. ;

Moldovan, A. ;

Dinescu, M. ;

Vasiliu, C. .

APPLIED SURFACE SCIENCE, 2007, 253 (19) :7711-7714

[6] Bacterial biofilms: A common cause of persistent infections [J].

Costerton, JW ;

Stewart, PS ;

Greenberg, EP .

SCIENCE, 1999, 284 (5418) :1318-1322

[7] Functionalized polysiloxane thin films deposited by matrix-assisted pulsed laser evaporation for advanced chemical sensor applications [J].

Houser, E. J. ;

Chrisey, D. B. ;

Bercu, M. ;

Scarisoreanu, N. D. ;

Purice, A. ;

Colceag, D. ;

Constantinescu, C. ;

Moldovan, A. ;

Dinescu, M. .

APPLIED SURFACE SCIENCE, 2006, 252 (13) :4871-4876

[8] Thin layers of bovine serum albumin by matrix assisted pulsed laser evaporation [J].

Jelinek, M. ;

Remsa, J. ;

Brynda, E. ;

Houska, A. ;

Kocourek, T. .

APPLIED SURFACE SCIENCE, 2007, 254 (04) :1240-1243

[9] MAPLE applications in studying organic thin films [J].

Jelinek, M. ;

Kocourek, T. ;

Remsa, J. ;

Cristescu, R. ;

Mihailescu, I. N. ;

Chrisey, D. B. .

LASER PHYSICS, 2007, 17 (02) :66-70

[10] Orientation of tetragonal lysozyme crystals nucleated on fatty acid thin films [J].

Kubo, T ;

Uchiyama, Y ;

Mizushima, T ;

Hondoh, H ;

Nakada, T .

JOURNAL OF CRYSTAL GROWTH, 2004, 269 (2-4) :535-541

← 1 2 3 4 →