Simultaneous Determination of Propranolol Hydrochloride and Sodium Benzoate in Oral Liquid Preparations by HPLC

被引:11
|
作者
Zahalka, L. [1 ]
Matysova, L. [1 ]
Sklubalova, Z. [2 ]
Klovrzova, S. [2 ,3 ]
Solich, P. [1 ]
机构
[1] Charles Univ Prague, Fac Pharm Hradec Kralove, Dept Analyt Chem, Hradec Kralove 50005, Czech Republic
[2] Charles Univ Prague, Fac Pharm, Dept Pharmaceut Technol, Hradec Kralove 50005, Czech Republic
[3] Gen Hosp Motol, Hosp Pharm, Prague, Czech Republic
关键词
HPLC; Propranolol hydrochloride; Sodium benzoate; Paediatric oral formulations; CHROMATOGRAPHY; VALIDATION; ATENOLOL; ASSAY;
D O I
10.1007/s10337-013-2457-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, selective and sensitive HPLC-UV method for quantification of propranolol hydrochloride and sodium benzoate in oral liquid preparations was developed and fully validated. Separation was performed by Supelco Discovery(A (R)) C18 (25 cm x 4.6 mm, particles 5 mu m) column. UV/VIS absorbance detector was set at wavelength 230 nm. Column oven was conditioned to 25 A degrees C. Mobile phase was prepared by dissolving 1.6 g of sodium dodecyl sulphate and 0.31 g of tetrabutylammonium dihydrogen phosphate in 450 mL of ultrapure water; 1 mL of sulphuric acid (95-97 %) and 550 mL of acetonitrile were added. Sodium hydroxide solution (2.1 M) was used for adjusting pH to value 3.3 (+/- 0.05). Retention times of sodium benzoate, propranolol hydrochloride and butylparaben (internal standard) were 2.2, 3.3 and 4.1 min, respectively. Newly developed method is suitable for simultaneous determination of propranolol hydrochloride and sodium benzoate in oral liquid preparations which are used for therapy of haemangiomas in paediatric patients. Method has been applied for stability testing of extemporaneous paediatric oral formulations containing propranolol hydrochloride.
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页码:1553 / 1558
页数:6
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