Performance Evaluation of Charged Aerosol and Evaporative Light Scattering Detection for the Determination of Ginsenosides by LC

被引：10
作者：
Wang, Li [1 ,2 ]
He, Wen-Shun [1 ]
Yan, Hai-Xia [1 ,2 ]
Jiang, Yong [1 ]
Bi, Kai-Shun [2 ]
Tu, Peng-Fei [1 ,2 ]
机构：
[1] Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
[2] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
来源：
CHROMATOGRAPHIA | 2009年 / 70卷 / 3-4期
基金：
中国国家自然科学基金;
关键词：
Column liquid chromatography-charged aerosol detection; Evaporative light scattering detection; UV detection; Ginsenosides; PRESSURIZED LIQUID EXTRACTION; PANAX-NOTOGINSENG; SHENMAI-INJECTION; DIFFERENT PARTS; HPLC-ELSD; CHROMATOGRAPHY; SAPONINS; GINSENG; IDENTIFICATION; ULTRAVIOLET;
D O I：
10.1365/s10337-009-1192-y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive LC-CAD method was developed for simultaneous determination of seven major triterpenoid saponins, namely ginsenosides Rg(1), Re, Rb-1, Rc, Rb-2, Rb-3 and Rd in Panax ginseng C. A. Meyer, a commonly used traditional Chinese medicine. This CAD method was evaluated in sensitivity, linearity and reproducibility compared to ELSD and UV. It was found the developed method has improved sensitivity, linearity and reproducibility compared to ELSD. This method was successfully applied to analyze the ginsenosides in ten samples of Panax ginseng. The validation results indicated that the improved method can be utilized as another approach for quality control of P. ginseng.
引用
收藏
页码：603 / 608
页数：6
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