Zamilon, a Novel Virophage with Mimiviridae Host Specificity

被引：84

作者：

Gaia, Morgan ^{[1
]}

Benamar, Samia ^{[1
]}

Boughalmi, Mondher ^{[1
]}

Pagnier, Isabelle ^{[1
]}

Croce, Olivier ^{[1
]}

Colson, Philippe ^{[1
,2
]}

Raoult, Didier ^{[1
,2
]}

La Scola, Bernard ^{[1
,2
]}

机构：

[1] Aix Marseille Univ, Unite Rech Malad Infect & Trop Emergentes, UMR CNRS IRD 7278, IFR48,Fac Med, Marseille, France

[2] Assistance Publ Hop Marseille, Pole Malad Infect, Marseille, France

来源：

PLOS ONE | 2014年 / 9卷 / 04期

关键词：

GIANT VIRUSES; DNA VIRUSES; GENOMES; PHYLOGENIES; HIGHLIGHTS; EVOLUTION; FAMILIES; SPUTNIK; ORIGIN;

D O I：

10.1371/journal.pone.0094923

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Virophages, which are potentially important ecological regulators, have been discovered in association with members of the order Megavirales. Sputnik virophages target the Mimiviridae, Mavirus was identified with the Cafeteria roenbergensis virus, and virophage genomes reconstructed by metagenomic analyses may be associated with the Phycodnaviridae. Despite the fact that the Sputnik virophages were isolated with viruses belonging to group A of the Mimiviridae, they can grow in amoebae infected by Mimiviridae from groups A, B or C. In this study we describe Zamilon, the first virophage isolated with a member of group C of the Mimiviridae family. By co-culturing amoebae with purified Zamilon, we found that the virophage is able to multiply with members of groups B and C of the Mimiviridae family but not with viruses from group A. Zamilon has a 17,276 bp DNA genome that potentially encodes 20 genes. Most of these genes are closely related to genes from the Sputnik virophage, yet two are more related to Megavirus chiliensis genes, a group B Mimiviridae, and one to Moumouvirus monve transpoviron.

引用

页数：8

共 48 条

[1] Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae [J].