Hyaluronic Acid-Povidone-Iodine Compound Facilitates Diabetic Wound Healing in a Streptozotocin-Induced Diabetes Rodent Model

被引：28

作者：

Chen, Rong-Fu ^{[1
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Wang, Chun-Ting ^{[1
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Chen, Yu-Hua ^{[1
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Chien, Ching-Ming ^{[1
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Lin, Sin-Daw ^{[1
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Lai, Chung-Sheng ^{[1
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Wang, Ching-Jen ^{[1
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Kuo, Yur-Ren ^{[1
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]}

机构：

[1] Kaohsiung Med Univ Hosp, Dept Surg, Div Plast Surg, 100 Tzyou 1st Rd, Kaohsiung 80756, Taiwan

[2] Ind Technol Res Inst, Dept Healthcare Mat, Mat & Chem Res Labs, Hsinchu, Taiwan

[3] Kaohsiung Chang Gung Mem Hosp, Dept Orthopaed, Kaohsiung, Taiwan

[4] Kaohsiung Med Univ, Fac Med, Coll Med, Orthopaed Res Ctr, Kaohsiung, Taiwan

[5] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung, Taiwan

来源：

PLASTIC AND RECONSTRUCTIVE SURGERY | 2019年 / 143卷 / 05期

关键词：

TISSUE-INJURY; THERAPY; SKIN; REGENERATION; ANTISEPSIS;

D O I：

10.1097/PRS.0000000000005504

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: This study investigated whether a hyaluronic acid-povidone-iodine compound can enhance diabetic wound healing. Methods: A dorsal skin defect (6 x 5 cm) in a streptozotocin-induced diabetes rodent model was used. Seventy male Wistar rats were divided into seven groups: I, normal control; II, diabetic control, no treatment; III, diabetic rats, lower molecular weight (100 kDa) hyaluronic acid; IV, rats, higher molecular weight (1000 kDa) hyaluronic acid; V, rats, 0.1% povidone-iodine; VI, rats, lower molecular weight hyaluronic acid plus povidone-iodine; and VII, rats, higher molecular weight hyaluronic acid plus povidone-iodine. Histologic examination was performed with hematoxylin and eosin staining. CD45, Ki-67, prolyl 4-hydroxylase, and vascular endothelial growth factor were evaluated with immunohistochemical staining. Results: Compared with the control, higher molecular weight hyaluronic acid plus povidone-iodine-treated rats had significantly reduced wound area (p < 0.001). Higher molecular weight hyaluronic acid plus povidone-iodine increased wound healing time when compared with higher molecular weight hyaluronic acid, povidone-iodine, or lower molecular weight hyaluronic acid plus povidone-iodine. Histology revealed significantly increased neovessels and suppressed inflammatory response in the higher molecular weight hyaluronic acid plus povidone-iodine group when compared with the control group. Immunohistochemical staining revealed significantly increased Ki67, prolyl 4-hydroxylase, and vascular endothelial growth factor expression, and suppressed CD45 expression in the higher molecular weight hyaluronic acid plus povidone-iodine group when compared with the other groups. Conclusion: Higher molecular weight hyaluronic acid plus povidone-iodine complex dressing significantly facilitated diabetic wound healing via increasing neovascularization and tissue regeneration and suppressing a proinflammatory response.

引用

页码：1371 / 1382

页数：12

共 25 条

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