Transforming growth factor-β1 is responsible for maturation-dependent spontaneous apoptosis of cultured gastric pit cells

被引：13

作者：

Tsutsumi, S ^{[1
]}

Tomisato, W ^{[1
]}

Hoshino, T ^{[1
]}

Tsuchiya, T ^{[1
]}

Mizushima, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Okayama 7008530, Japan

来源：

EXPERIMENTAL BIOLOGY AND MEDICINE | 2002年 / 227卷 / 06期

关键词：

apoptosis; gastric mucosal cells; caspases; transforming growth factor-beta 1;

D O I：

10.1177/153537020222700606

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

In this study, we established a system of high concentration serum-dependent spontaneous apoptosis of guinea pig gastric pit cells in primary culture, which seems to mimic the spontaneous apoptosis of matured gastric pit cells at gastric surface in vivo. In addition to induction of the spontaneous apoptosis, cell growth was inhibited in the presence of 10% serum compared with 0.5% serum. Transforming growth factor-beta1 (TGF-beta1), which is known to cause both apoptosis and growth inhibition in mammalian cells, was present in serum of both fetal calf and guinea pig. The addition of recombinant TGF-beta1 to the culture medium containing 0.5% fetal calf serum caused both induction of apoptosis and inhibition of cell growth. On the other hand, immunodepletion of TGF-beta1 from fetal calf serum caused inability to induce both the spontaneous apoptosis and inhibition of cell growth. These data suggest that TGF-beta1 is involved in the spontaneous apoptosis of guinea pig gastric pit cells in primary culture.

引用

页码：402 / 411

页数：10

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