Vimentin intermediate filament and plectin provide a scaffold for invadopodia, facilitating cancer cell invasion and extravasation for metastasis

被引：88

作者：

Yoneyama, Mihoko Sutoh ^{[1
]}

Hatakeyama, Shingo ^{[1
]}

Habuchi, Tomonori ^{[2
]}

Inoue, Takamitsu ^{[2
]}

Nakamura, Toshiya ^{[3
]}

Funyu, Tomihisa ^{[4
]}

Wiche, Gerhard ^{[5
]}

Ohyama, Chikara ^{[1
]}

Tsuboi, Shigeru ^{[1
,4
]}

机构：

[1] Hirosaki Univ, Grad Sch Med, Dept Urol, Hirosaki, Aomori, Japan

[2] Akita Univ, Grad Sch Med, Dept Urol, Akita 0108543, Japan

[3] Hirosaki Univ, Grad Sch Hlth Sci, Dept Biomed Sci, Hirosaki, Aomori, Japan

[4] Oyokyo Kidney Res Inst, Dept Canc Immunol & Cell Biol, Hirosaki, Aomori, Japan

[5] Univ Vienna, Dept Biochem & Cell Biol, Max F Perutz Labs, A-1030 Vienna, Austria

来源：

EUROPEAN JOURNAL OF CELL BIOLOGY | 2014年 / 93卷 / 04期

关键词：

Cancer cell extravasation; Invadopodium; Invadosome; Invasion; Plectin; Vimentin; EPITHELIAL-MESENCHYMAL TRANSITION; PODOSOME FORMATION; IN-VIVO; F-ACTIN; MIGRATION; CORTACTIN; BINDING; MECHANISMS; ADHESION;

D O I：

10.1016/j.ejcb.2014.03.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate the molecular mechanisms of cancer metastasis, we have isolated a high-metastatic bladder cancer cell subpopulation from a low-metastatic cell line by using an in vivo selection system. Cells in the subpopulation showed a high ability to form invadopodia, the filamentous actin (F-actin)-based membrane protrusions that play an essential role in cancer cell invasion. Analysis of the gene expression profile revealed that the expression of an intermediate filament (IF) protein, vimentin and a cytoskeletal linker protein, plectin was up-regulated in the high-metastatic subpopulation compared with the low metastatic cell line. Here we report a novel role of vimentin IF and plectin in metastasis. In invasive bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link was formed. Disruption of this link severely impaired invadopodia formation, reducing the capacities of extracellular matrix degradation, transendothelial migration and metastasis. In addition, the vimentin assembly into the filaments was required for invadopodia formation. Our results suggest that plectin anchoring invadopodia to vimentin IF scaffolds and stabilizes invadopodia, which is a critical molecular process for cancer cell invasion and extravasation for metastasis. (C) 2014 Elsevier GmbH. All rights reserved.

引用

页码：157 / 169

页数：13

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