Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells

被引：38

作者：

Monetti, Emanuela ^{[1
,2
]}

Kadono, Takashi ^{[1
,3
,4
]}

Tran, Daniel ^{[1
,2
]}

Azzarello, Elisa

Arbelet-Bonnin, Delphine ^{[1
,2
]}

Biligui, Bernadette ^{[1
,2
]}

Briand, Joel ^{[1
,2
]}

Kawano, Tomonori ^{[3
,5
,6
]}

Mancuso, Stefano ^{[5
,6
]}

Bouteau, Francois ^{[1
,2
,5
]}

机构：

[1] Univ Paris Diderot, Sorbonne Paris Cite, Inst Energies Demain UMR8236, Paris, France

[2] Inst Biol Plantes, F-91405 Orsay, France

[3] Univ Kitakyushu 1 1, Grad Sch Environm Engn, Wakamatsu Ku, Kitakyushu, Fukuoka 8080135, Japan

[4] Kyushu Univ, Fac Agr, Lab Crop Sci, Dept Plant Resources,Higashi Ku, Fukuoka 8128581, Japan

[5] Univ Florence, LINV Kitakyushu Res Ctr LINV Kitakyushu, Kitakyushu, Fukuoka, Japan

[6] Univ Paris Diderot, Sorbonne Paris Cite, Paris Interdisciplinary Energy Res Inst PIERI, Paris, France

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2014年 / 65卷 / 05期

基金：

日本学术振兴会;

关键词：

Calcium; hyperosmotic stress; mitochondria; NaCl; Nicotiana tabacum; non-selective cation channels; programmed cell death; reactive oxygen species; NONSELECTIVE CATION CHANNELS; SALT STRESS; SINGLET-OXYGEN; POTENTIAL DIFFERENCE; SUSPENSION-CULTURE; CYTOSOLIC CALCIUM; STOMATAL CLOSURE; OSMOTIC-STRESS; H+ EXTRUSION; ARABIDOPSIS;

D O I：

10.1093/jxb/ert460

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hyperosmotic stresses represent one of the major constraints that adversely affect plants growth, development, and productivity. In this study, the focus was on early responses to hyperosmotic stress- (NaCl and sorbitol) induced reactive oxygen species (ROS) generation, cytosolic Ca-2 concentration ([Ca-2](cyt)) increase, ion fluxes, and mitochondrial potential variations, and on their links in pathways leading to programmed cell death (PCD). By using BY-2 tobacco cells, it was shown that both NaCl- and sorbitol-induced PCD seemed to be dependent on superoxide anion (O-2) generation by NADPH-oxidase. In the case of NaCl, an early influx of sodium through non-selective cation channels participates in the development of PCD through mitochondrial dysfunction and NADPH-oxidase-dependent O-2 generation. This supports the hypothesis of different pathways in NaCl- and sorbitol-induced cell death. Surprisingly, other shared early responses, such as [Ca-2](cyt) increase and singlet oxygen production, do not seem to be involved in PCD.

引用

页码：1361 / 1375

页数：15

共 64 条

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