Recombinant human tyrosyl-tRNA synthetase, a novel thrombopoietic agent

被引：2
|
作者：
Zhan Ling [1 ,2 ]
Zhang Yanling [1 ,2 ]
Feng Zhe [1 ,2 ]
Chen Kui [1 ,2 ]
Zhou Xiushi [1 ,2 ]
Yu Min [1 ,2 ]
Mo Wei [1 ,2 ]
机构：
[1] Fudan Univ, Minist Educ, Key Lab Mol Med, Shanghai 200032, Peoples R China
[2] Fudan Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Shanghai 200032, Peoples R China
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY | 2014年 / 738卷
基金：
中国国家自然科学基金;
关键词：
Recombinant human tyrosyl; tRNA synthetase; Thrombocytopenia; Cyclophosphamide; Aggregation of megakaryocytes; Vascular cell adhesion molecule; BONE-MARROW; ENDOTHELIAL-CELLS; CYCLOPHOSPHAMIDE; ACTIVATION; REGULATOR; CYTOKINES; PATHWAYS; PECAM-1;
D O I：
10.1016/j.ejphar.2014.05.054
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
chemo- and radiotherapy induced thrombocytopenia present significant limitations for tumor therapy. In this study, we examined the therapeutic effect of recombinant human tyrosyl-tRNA synthetase (rhTyrRS) against development of thrombocytopenia in cyclophosphamide (CTX) treated mice. Our data indicate that intraperitoneal administration of at least 10 mu g/day rhTyrRS for 7 days significantly prevents the decrease in platelets 8 days after CTX injection. The data further demonstrate that rhTyrRS promotes migration and aggregation of megakaryocytes to the bone marrow niche. Real-time PCR and flow cytometry reveal that VCAM-1 is particularly important for the adhesion. Together, these data suggest that rhTyrRS might be a useful therapeutic agent for chemotherapy-induced thrombocytopenia. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：293 / 300
页数：8
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