Characterization of a novel rice gene OsATX and modulation of its expression by components of the stress signalling pathways

被引:16
作者
Agrawal, GK
Rakwal, R
Jwa, NS
Agrawal, VP
机构
[1] Res Lab Agr Biotechnol & Biochem, Kathmandu, Nepal
[2] Japan Sci & Technol Corp, Tokyo, Japan
[3] Sejong Univ, Coll Nat Sci, Dept Mol Biol, Seoul 143747, South Korea
关键词
D O I
10.1034/j.1399-3054.2002.1160111.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In our search to identify gene(s) involved in the rice self-defense responses, we cloned a novel rice (Oryza sativa L. cv. Nipponbare) gene, OsATX , a single copy gene, from the JA treated rice seedling leaves cDNA library. This gene encodes a 69 amino acid polypeptide with a predicted molecular mass of 7649.7 and a pI of 5.6. OsATX was responsive to cutting (wounding by cutting the excised leaf), over its weak constitutive expression in the healthy leaves. The critical signalling molecules, jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and hydrogen peroxide, together with protein phosphatase inhibitors, effectively up-regulated the OsATX expression with time, over the excised leaf cut control, whereas ethylene had no affect. Furthermore, copper, a heavy metal, also up-regulated OsATX expression. Moreover, induced expression of OsATX mRNA was influenced by light signal(s), and showed a requirement for de novo synthesized protein factors. Additionally, co-application of either JA or ABA with SA drastically suppressed the induced OsATX mRNA level. Finally, the blast pathogen, Magnaporthe grisea , triggered OsATX mRNA accumulation. These results strongly suggest a function/role(s) for OsATX in defense/stress responses in rice.
引用
收藏
页码:87 / 95
页数:9
相关论文
共 32 条
[1]   Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals [J].
Agrawal, GK ;
Rakwal, R ;
Jwa, NS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 278 (02) :290-298
[2]   A novel rice (Oryza sativa L.) acidic PR1 gene highly responsive to cut, phytohormones, and protein phosphatase inhibitors [J].
Agrawal, GK ;
Jwa, NS ;
Rakwal, R .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (01) :157-165
[3]   Signalling molecules and blast pathogen attack activates rice OsPR1a and OsPR1b genes:: A model illustrating components participating during defence/stress response [J].
Agrawal, GK ;
Rakwal, R ;
Jwa, NS ;
Agrawal, VP .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2001, 39 (12) :1095-1103
[4]   Differential induction of three pathogenesis-related genes, PR10, PR1b and PR5 by the ethylene generator ethephon under light and dark in rice (Oryza sativa L.) seedlings [J].
Agrawal, GK ;
Rakwal, R ;
Jwa, NS .
JOURNAL OF PLANT PHYSIOLOGY, 2001, 158 (01) :133-137
[5]   Salicylic acid and disease resistance in plants [J].
Dempsey, DA ;
Shah, J ;
Klessig, DF .
CRITICAL REVIEWS IN PLANT SCIENCES, 1999, 18 (04) :547-575
[6]   Commercial sector scores success with whole rice genome [J].
Dickson, D ;
Cyranoski, D .
NATURE, 2001, 409 (6820) :551-551
[7]  
ECKER JR, 1995, SCIENCE, V268, P667, DOI 10.1126/science.7732375
[8]   Interplay of signaling pathways in plant disease resistance [J].
Feys, BJ ;
Parker, JE .
TRENDS IN GENETICS, 2000, 16 (10) :449-455
[9]   Salicylic acid response in rice:: influence of salicylic acid on H2O2 accumulation and oxidative stress [J].
Ganesan, V ;
Thomas, G .
PLANT SCIENCE, 2001, 160 (06) :1095-1106
[10]   Role of reactive oxygen intermediates and cognate redox signaling in disease resistance [J].
Grant, JJ ;
Loake, GJ .
PLANT PHYSIOLOGY, 2000, 124 (01) :21-29