Influence of Polyelectrolyte on DNA-RecA Nucleoprotein Filaments: Poly-L-Lysine Used as a Model

被引：2

作者：

Guo, Cunlan ^{[1
]}

Li, Gaiping ^{[1
]}

Liu, Zhelin ^{[1
]}

Sun, Lanlan ^{[1
]}

Sun, Yujing ^{[1
]}

Xu, Fugang ^{[1
]}

Zhang, Yue ^{[1
]}

Yang, Tao ^{[1
]}

Li, Zhuang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Grad Sch, Changchun 130022, Jilin, Peoples R China

来源：

CHEMPHYSCHEM | 2009年 / 10卷 / 9-10期

基金：

中国国家自然科学基金;

关键词：

atomic force microscopy; DNA; gel electrophoresis; nucleoprotein filaments; poly-L-lysine; SINGLE-STRANDED-DNA; DEPENDENT ATPASE ACTIVITY; ESCHERICHIA-COLI; DUPLEX DNA; BINDING-PROTEIN; RECOMBINATION; INTERCALATORS; COMPLEXES; MECHANISM; EXCHANGE;

D O I：

10.1002/cphc.200800850

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

RecA of Escherichia coli and its active nucleoprotein filaments with DNA are important for the genomic integrity and the genetic diversity. The formation of the DNA-RecA nucleoprotein filaments is a complex multiple-step process and can be affected by many factors. In this work, the effects of poly-L-lysine (PLL) on the DNA-RecA nucleoprotein filaments are investigated in vitro by agarose gel electrophoresis and atomic force microscopy (AFM). The observed morphologies vary with the concentration, the length, and the addition order of PLL. These distinctions provide information for the conformation change of DNA and the binding sites of RecA protein in the formation process of nucleoprotein filaments. Besides that, the comparison displays the effect of cationic polyelectrolyte on both inhibition and destabilization of the DNA-RecA nucleoprotein filaments. The current study provides valuable insights for pharmacologic studies and gene delivery.

引用

页码：1624 / 1629

页数：6

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