Highly selective and sensitive detection of histidine by naked eye and fluorimetric method in aqueous medium via hydrogen bonding

A new turn on fluorescent rhodamine dye primarily based probe was designed and synthesized. The dye suggests a sensitive detection of histidine at a variety of concentrations in aqueous medium by using fluorimetric method. The absorption and emission depth had been all at once changed upon the addition of histidine with probe, there is no change when addition of other competitors. The dye confirmed color change from colorless to pink during addition of histidine, which could be determined by naked eye. The Job's plot indicated that the 1:1 binding ratio of probe with analyte. The probe shows the detection limit of histidine is 1.67 nM. The binding of probe with histidine was proven through NMR titrations, mass and strips method.