Isolation and characterization of 27 novel polymorphic microsatellite markers in oriental river prawn Macrobrachium nipponense

被引：2
|
作者：
Zhao, Yan [1 ]
Wang, Hui [1 ]
Ji, XiangShan [1 ]
Yang, YuZhu [1 ]
Zeng, YongQing [1 ]
机构：
[1] Shandong Agr Univ, Coll Anim Sci & Technol, Tai An 271018, Shandong, Peoples R China
来源：
CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 02期
关键词：
Oriental river prawn; Microsatellite; FIASCO;
D O I：
10.1007/s12686-013-0129-9
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
We developed 27 polymorphic microsatellite loci by constructing a microsatellite-enriched genomic library. The number of alleles per locus ranged from two to seven, and observed and expected heterozygosities from 0.1905 to 0.8000 and from 0.1765 to 0.8017 respectively. Seven loci (X63, X195, X208, X348, X731, X795, X1214, and XB96) deviated from the Hardy-Weinberg equilibrium in the sampled population after BONFERRONI correction (adjusted P value = 0.003). These microsatellite markers will be useful for the study of population structure, genetic diversity and the construction of the genetic mapping of Macrobrachium nipponense.
引用
收藏
页码：293 / 295
页数：3
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