Peptide Fractions Obtained from Rice By-Products by Means of an Environment-Friendly Process Show In Vitro Health-Related Bioactivities

被引：37

作者：

Ferri, Maura ^{[1
]}

Graen-Heedfeld, Jurgen ^{[2
]}

Bretz, Karlheinz ^{[2
]}

Guillon, Fabien ^{[3
]}

Michelini, Elisa ^{[4
]}

Calabretta, Maria Maddalena ^{[4
]}

Lamborghini, Matteo ^{[5
]}

Gruarin, Nicolo ^{[1
]}

Roda, Aldo ^{[4
]}

Kraft, Axel ^{[2
]}

Tassoni, Annalisa ^{[1
]}

机构：

[1] Univ Bologna, Dept Biol Geol & Environm Sci, Bologna, Italy

[2] Fraunhofer Inst Environm Safety & Energy Technol, Oberhausen, Germany

[3] Sterlab, Cell Culture Lab, Vallauris, France

[4] Univ Bologna, Dept Chem G Ciamician, Bologna, Italy

[5] Carminia Snc, Bologna, Italy

来源：

PLOS ONE | 2017年 / 12卷 / 01期

关键词：

TYROSINASE INHIBITOR; ANTIOXIDANT PEPTIDES; CONVERTING-ENZYME; BRAN PROTEIN; IDENTIFICATION; POLYPHENOLS; ASSAY;

D O I：

10.1371/journal.pone.0170954

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recently, the isolation of new health-related bioactive molecules derived from agro-food industrial by-products by means of environment-friendly extraction processes has become of particular interest. In the present study, a protein by-product from the rice starch industry was hydrolysed with five commercial proteolytic enzymes, avoiding the use of solvents or chemicals. The digestion processes were optimised, and the digestates were separated in fractions with four different molecular weight ranges by using a cross-flow membrane filtration technique. Total hydrolysates and fractions were tested in vitro for a wide range of biological activities. For the first time rice-derived peptides were assayed for anti-tyrosinase, anti-inflammatory, cytotoxicity and irritation capacities. Antioxidant and anti-hypertensive activities were also evaluated. Protamex, Alcalase and Neutrase treatments produced peptide fractions with valuable bioactivities without resulting cytotoxic or irritant. Highest levels of bioactivity were detected in Protamex-derived samples, followed by samples treated with Alcalase. Based on the present results, a future direct exploitation of isolated peptide fractions in the nutraceutical, functional food and cosmetic industrial fields may be foreseen.

引用

页数：14

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