Influence of Phospholipid Composition on Self-Assembly and Energy-Transfer Efficiency in Networks of Light-Harvesting 2 Complexes

被引：30

作者：

Sumino, Ayumi ^{[1
]}

Dewa, Takehisa ^{[1
,2
]}

Noji, Tomoyasu ^{[1
]}

Nakano, Yuki ^{[1
]}

Watanabe, Natsuko ^{[1
]}

Hildner, Richard ^{[3
,4
]}

Boesch, Nils ^{[3
,4
]}

Koehler, Juergen ^{[3
,4
]}

Nango, Mamoru ^{[1
]}

机构：

[1] Nagoya Inst Technol, Grad Sch Engn, Dept Frontier Mat, Showa Ku, Nagoya, Aichi 4668555, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

[3] Univ Bayreuth, D-95440 Bayreuth, Germany

[4] Univ Bayreuth, BIMF, D-95440 Bayreuth, Germany

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2013年 / 117卷 / 36期

基金：

日本科学技术振兴机构;

关键词：

ELECTRONICALLY EXCITED-STATES; TIME-RESOLVED SPECTROSCOPY; LH2; COMPLEXES; MEMBRANE-PROTEIN; RHODOPSEUDOMONAS-PALUSTRIS; PHOTOSYNTHETIC APPARATUS; RHODOSPIRILLUM-RUBRUM; CRYSTAL-STRUCTURE; PURPLE BACTERIA; ACIDOPHILA;

D O I：

10.1021/jp4047819

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In the photosynthetic membrane of purple bacteria networks of light-harvesting 2 (LH2) complexes capture the sunlight and transfer the excitation energy. In order to investigate the mutual relationship between the supramolecular organization of the pigment-protein complexes and their biological function, the LH2 complexes were reconstituted into three types of phospholipid membranes, consisting of L-alpha-phosphatidylglycerol (PG), L-alpha-phosphatidylcholine (PC), and L-alpha-phosphatidylethanolamine (PE)/PG/cardiolipin (CL). Atomic force microscopy (AFM) revealed that the type of phospholipids had a crucial influence on the clustering tendency of the LH2 complexes increased from PG over PC to PE/PG/CL, where the LH2 complexes formed large, densely packed clusters. Time-resolved spectroscopy uncovered a strong quenching of the LH2 fluorescence that is ascribed to singlet-singlet and singlet-triplet annihilation by an efficient energy transfer between the LH2 complexes in the artificial membrane systems. Quantitative analysis reveals that the intercomplex energy transfer efficiency varies strongly as a function of the morphology of the nanostructure, namely in the order PE/PG/CL > PC > PG, which is in line with the clustering tendency of LH2 observed by AFM. These results suggest a strong influence of the phospholipids on the self-assembly of LH2 complexes into networks and concomitantly on the intercomplex energy transfer efficiency.

引用

页码：10395 / 10404

页数：10

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