Fermentation, biodegradation and tensile strength of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) synthesized by Delftia acidovorans

被引：11

作者：

Hsieh, Wen-Chuan ^{[1
]}

Wada, Yuki ^{[2
]}

Chang, Chih-Pong ^{[3
]}

机构：

[1] I Shou Univ, Dept Biol Sci & Technol, Kaohsiung 82445, Taiwan

[2] Gunma Univ, Dept Biol & Chem Engn, Gunma 3768515, Japan

[3] Chinese Culture Univ, Dept Text Engn, Taipei 11192, Taiwan

来源：

JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS | 2009年 / 40卷 / 02期

关键词：

Fermentation; Biodegradation; Tensile strength; P(3HB-co-4HB); Poly(3-hydroxybutyrate-co-4-hydroxybutyrate); COMAMONAS-ACIDOVORANS; ALCALIGENES-EUTROPHUS; POLY(4-HYDROXYBUTYRIC ACID); HYDROGENOPHAGA-PSEUDOFLAVA; MICROBIAL SYNTHESIS; ESCHERICHIA-COLI; BIOSYNTHESIS; DEGRADATION; SCAFFOLDS; CELLS;

D O I：

10.1016/j.jtice.2008.11.004

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

This study discusses poly(3-hydroxybutyrate-co-4-hydroxybutyrate) that is produced by Delftia acidovorans bacteria in fed-batch cultures from various carbon sources. The experimentally determined optimal fed-batch culture conditions are as follows: pH: 7.0, incubation temperature: 26 degrees C, incubation time: 72 h, and concentration of carbon source: 10 g/L. The maximum 4HB unit composition of P(3HB-co-4HB) obtained using the fed-batch culture with a cell concentration of about 2.5 g/L was 94 mol% although the P(3HB-co-4HB) content remained low, at around 13 wt%. The extent of the enzymatic degradation of P(3HB-co-4HB) was determined by involved PHB depolymerase and lipase. The tensile strength of P(3HB-co-4HB) markedly decreased during 2 h of degradation by PHB depolymerase, whereas that of P(3HB-co-4HB) degraded by lipase for 50 h was at least 3 MPa. (C) 2008 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

引用

页码：143 / 147

页数：5