Investigation of doxorubicin resistance in MCF-7 breast cancer cells using shot-gun comparative proteomics with proteolytic 18O labeling

被引：64
作者：
Brown, KJ [1 ]
Fenselau, C [1 ]
机构：
[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
来源：
JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 03期
关键词：
proteolytic labeling; drug resistance; breast cancer cells;
D O I：
10.1021/pr0340835
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A shot-gun comparative proteomic investigation utilizing proteolytic O-18 labeling has been carried out on a drug susceptible MCF-7 human breast cancer cell line and a related cell line that is resistant to doxorubicin. The proteolytic O-18 labeling method has been further refined and optimized for application to a protein fraction stemming from the cytosol of the breast cancer cells. The comparative investigation revealed several proteins with altered expression levels in the doxorubicin resistant line. These altered proteins are considered for a possible role in doxorubicin resistance.
引用
收藏
页码：455 / 462
页数：8
相关论文
共 76 条
  • [1] Alaiya AA, 2000, ELECTROPHORESIS, V21, P1210, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1210::AID-ELPS1210>3.3.CO
  • [2] 2-J
  • [3] Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cells
    Aoudjit, F
    Vuori, K
    [J]. ONCOGENE, 2001, 20 (36) : 4995 - 5004
  • [4] Arts HJG, 1999, CLIN CANCER RES, V5, P2798
  • [5] Asaumi J, 1996, ANTICANCER RES, V16, P247
  • [6] Badock V, 2001, ELECTROPHORESIS, V22, P2856, DOI 10.1002/1522-2683(200108)22:14<2856::AID-ELPS2856>3.0.CO
  • [7] 2-U
  • [8] Bates SE, 1999, CLIN CANCER RES, V5, P3346
  • [9] Global internal standard technology for comparative proteomics
    Chakraborty, A
    Regnier, FE
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2002, 949 (1-2) : 173 - 184
  • [10] Triggering and modulation of apoptosis by oxidative stress
    Chandra, J
    Samali, A
    Orrenius, S
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2000, 29 (3-4) : 323 - 333
12345678