Rat model of veno-arterial extracorporeal membrane oxygenation

被引：21

作者：

Ali, Ayyaz A. ^{[1
,2
]}

Downey, Peter ^{[1
,2
]}

Singh, Gopal ^{[1
,2
]}

Qi, Wei ^{[1
,2
]}

George, Isaac ^{[1
,2
]}

Takayama, Hiroo ^{[1
,2
]}

Kirtane, Ajay ^{[1
,2
,3
]}

Krishnan, Prakash ^{[4
]}

Zalewski, Adrian ^{[1
,2
]}

Freed, Darren ^{[8
,9
]}

Large, Stephen R. ^{[6
,7
]}

Ashley, Euan A. ^{[5
]}

Leon, Martin B. ^{[1
,2
,3
]}

Bacchetta, Matthew ^{[1
,2
]}

Ali, Ziad A. ^{[1
,2
,3
]}

机构：

[1] New York Presbyterian Hosp, Ctr Intervent Vasc Therapy, Div Cardiol, New York, NY 10032 USA

[2] Columbia Univ, New York, NY 10032 USA

[3] Cardiovasc Res Fdn, New York, NY USA

[4] Mt Sinai Sch Med, Zena & Michael A Weiner Cardiovasc Inst, New York, NY 10029 USA

[5] Stanford Univ, Div Cardiovasc Med, Stanford, CA 94305 USA

[6] Papworth Hosp, Dept Cardiothorac Surg, Cambridge CB23 3RE, England

[7] Univ Cambridge, Cambridge CB23 3RE, England

[8] Univ Alberta, Edmonton, AB T6G 2P4, Canada

[9] Mazankowski Alberta Heart Inst, Edmonton, AB T6G 2P4, Canada

来源：

JOURNAL OF TRANSLATIONAL MEDICINE | 2014年 / 12卷

关键词：

ECMO; Extracorporeal membrane oxygenation; Resuscitation; Cardiac arrest;

D O I：

10.1186/1479-5876-12-37

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: We aim to develop a rat model of veno-arterial extracorporeal membrane oxygenation (VA-ECMO). Methods: VA-ECMO was established in twelve Male Sprague-Dawley rats (250-350 g) through cannulation of the right jugular vein for venous drainage and the right femoral artery for arterial reinfusion. Arterial blood pressure was measured using a conductance catheter through cannulation of the left carotid artery. Heart rate was monitored by electrocardiography and arterial blood gas parameters with a blood gas analyzer. The VA-ECMO circuit was tested by subjecting the rats to hypoxic cardiac arrest with resuscitation using VA-ECMO. Both load-dependent and load-independent measures of myocardial contractility were measured using pressure-volume loop analysis to confirm restoration of myocardial function post-resuscitation. Results: Following hypoxic cardiac arrest VA-ECMO provided sufficient oxygenation to support the circulation. The haemodynamic and blood gas parameters were maintained at transition and during ECMO. All animals were resuscitated, regained cardiac function and were able to be weaned off ECMO post-resuscitation. Conclusion: We have established a safe, high-throughput, economical, functioning rat model of VA-ECMO.

引用

页数：6

共 5 条

[1] A 5-year experience with cardiopulmonary resuscitation using extracorporeal life support in non-postcardiotomy patients with cardiac arrest [J].