A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

被引：70
作者：
Cassimjee, K. Engelmark [1 ]
Kadow, M. [1 ]
Wikmark, Y. [1 ]
Humble, M. Svedendahl [1 ]
Rothstein, M. L. [2 ]
Rothstein, D. M. [2 ]
Backvall, J. -E. [1 ]
机构：
[1] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, SE-10691 Stockholm, Sweden
[2] Prime Synth Inc, Aston, PA 19014 USA
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 65期
基金：
瑞典研究理事会;
关键词：
ENZYME IMMOBILIZATION; OMEGA-TRANSAMINASE; RATIONAL REDESIGN; POROUS GLASSES; LIPASE-B; AMINATION; BINDING; KETONES; REVEAL;
D O I：
10.1039/c4cc02605e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
引用
收藏
页码：9134 / 9137
页数：4
相关论文
共 36 条
  • [21] Evaluation of immobilized enzymes for industrial applications
    Liese, Andreas
    Hilterhaus, Lutz
    [J]. CHEMICAL SOCIETY REVIEWS, 2013, 42 (15) : 6236 - 6249
  • [22] An S-selective lipase was created by rational redesign and the enantioselectivity increased with temperature
    Magnusson, AO
    Takwa, M
    Harnberg, A
    Hult, K
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (29) : 4582 - 4585
  • [23] Creating space for large secondary alcohols by rational redesign of Candida antarctica lipase B
    Magnusson, AO
    Rotticci-Mulder, JC
    Santagostino, A
    Hult, K
    [J]. CHEMBIOCHEM, 2005, 6 (06) : 1051 - 1056
  • [24] ON THE INTERFACIAL ACTIVATION OF CANDIDA-ANTARCTICA LIPASE-A AND LIPASE-B AS COMPARED WITH HUMICOLA-LANUGINOSA LIPASE
    MARTINELLE, M
    HOLMQUIST, M
    HULT, K
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1995, 1258 (03): : 272 - 276
  • [25] Asymmetric Bio-amination of Ketones in Organic Solvents
    Mutti, Francesco G.
    Kroutil, Wolfgang
    [J]. ADVANCED SYNTHESIS & CATALYSIS, 2012, 354 (18) : 3409 - 3413
  • [26] Amination of Ketones by Employing Two New (S)-Selective ω-Transaminases and the His-Tagged ω-TA from Vibrio fluvialis
    Mutti, Francesco G.
    Fuchs, Christine S.
    Pressnitz, Desiree
    Turrini, Nikolaus G.
    Sattler, Johann H.
    Lerchner, Alexandra
    Skerra, Arne
    Kroutil, Wolfgang
    [J]. EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2012, 2012 (05) : 1003 - 1007
  • [27] Pon Richard T., 1993, V20, P465
  • [28] Rothstein M. L., 2008, US Pat., Patent No. [12 145 051, 12145051]
  • [29] Crystallization and preliminary X-ray diffraction analysis of ω-amino acid:pyruvate transaminase from Chromobacterium violaceum
    Sayer, Christopher
    Isupov, Michail N.
    Littlechild, Jennifer A.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2007, 63 : 117 - 119
  • [30] Towards practical biocatalytic Baeyer-Villiger reactions: applying a thermostable enzyme in the gram-scale synthesis of optically-active lactones in a two-liquid-phase system
    Schulz, Frank
    Leca, Francois
    Hollmann, Frank
    Reetz, Manfred T.
    [J]. BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY, 2005, 1
1234