A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

被引：70
作者：
Cassimjee, K. Engelmark [1 ]
Kadow, M. [1 ]
Wikmark, Y. [1 ]
Humble, M. Svedendahl [1 ]
Rothstein, M. L. [2 ]
Rothstein, D. M. [2 ]
Backvall, J. -E. [1 ]
机构：
[1] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, SE-10691 Stockholm, Sweden
[2] Prime Synth Inc, Aston, PA 19014 USA
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 65期
基金：
瑞典研究理事会;
关键词：
ENZYME IMMOBILIZATION; OMEGA-TRANSAMINASE; RATIONAL REDESIGN; POROUS GLASSES; LIPASE-B; AMINATION; BINDING; KETONES; REVEAL;
D O I：
10.1039/c4cc02605e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.
引用
收藏
页码：9134 / 9137
页数：4
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