A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

被引：71

作者：

Cassimjee, K. Engelmark ^{[1
]}

Kadow, M. ^{[1
]}

Wikmark, Y. ^{[1
]}

Humble, M. Svedendahl ^{[1
]}

Rothstein, M. L. ^{[2
]}

Rothstein, D. M. ^{[2
]}

Backvall, J. -E. ^{[1
]}

机构：

[1] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, SE-10691 Stockholm, Sweden

[2] Prime Synth Inc, Aston, PA 19014 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 65期

基金：

瑞典研究理事会;

关键词：

ENZYME IMMOBILIZATION; OMEGA-TRANSAMINASE; RATIONAL REDESIGN; POROUS GLASSES; LIPASE-B; AMINATION; BINDING; KETONES; REVEAL;

D O I：

10.1039/c4cc02605e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

引用

页码：9134 / 9137

页数：4

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