Exon mapping by fiber-FISH or LR-PCR

被引：28

作者：

Florijn, RJ

vandeRijke, FM

Vrolijk, H

Blonden, LAJ

Hofker, MH

denDunnen, JT

Tanke, HJ

vanOmmen, GJB

Raap, AK

机构：

[1] LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,NL-2333 AL LEIDEN,NETHERLANDS

[2] LEIDEN STATE UNIV,DEPT HUMAN GENET,NL-2333 AL LEIDEN,NETHERLANDS

来源：

GENOMICS | 1996年 / 38卷 / 03期

关键词：

D O I：

10.1006/geno.1996.0629

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of greater than or equal to 200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations. (C) 1996 Academic Press, Inc.

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页码：277 / 282

页数：6

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