The distribution of podophyllotoxin in the different parts of Dysosma versipellis (Hance) M.!Cheng by capillary zone electrophoresis

A simple and sensitive capillary zone electrophoresis (CZE) method for the determination of podophyllotoxin (PPT) has been developed, and the distribution of podophyllotoxin in the different part of Dysosma versipellis (Hance) M. Cheng was studied by the proposed method. The results indicated that podophyllotoxin could be separated availably from the other constituents in the different part of Dysosma versipellis ( Hance) M. Cheng in an uncoated fused-silica capillary (57 cm X 75 mu m i. d. with the effective length of 50 cm) using 25 mmol/L borate with pH 10.8 as running buffer. The linear range for the determination of podophyllotoxin was 1.5 - 171.0 mg/L with the relative standard deviations (n = 8) for the relative migration time and peak area of 1.5% and 0.8%, respectively. The results obtained from the determination of podophyllotoxin in the ethanolic extracts of the roots, stems, leaves and fibrils of Dysosma versipellis (Hance) M. Cheng pointed out that the distribution of podophyllotoxin in the different part of Dysosma versipellis ( Hance) M. Cheng was not equal. Podophyllotoxin in the roots was highest, and in the leaves was lowest.