Rational Design of Self-Assembled Cationic Porphyrin-Based Nanoparticles for Efficient Photodynamic Inactivation of Bacteria

被引:61
作者
Li, Junying [1 ]
Sun, Wei [1 ]
Yang, Zihuayuan [1 ]
Gao, Ge [1 ]
Ran, Huan-Huan [1 ]
Xu, Ke-Fei [1 ]
Duan, Qiu-Yi [1 ]
Liu, Xiaoyang [1 ]
Wu, Fu-Gen [1 ]
机构
[1] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China
基金
中国国家自然科学基金;
关键词
porphyrin; photosensitizer; antimicrobial; bacterial inactivation; bacterial infection; GRAM-NEGATIVE BACTERIA; PHOTOINACTIVATION; PHOTOSENSITIZERS; PHTHALOCYANINES; NANOSHEETS; DISCOVERY;
D O I
10.1021/acsami.0c15244
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Bacterial infection has become an urgent health problem in the world. Especially, the evolving resistance of bacteria to antibiotics makes the issue more challenging, and thus new treatments to fight these infections are needed. Antibacterial photodynamic therapy (aPDT) is recognized as a novel and promising method to inactivate a wide range of bacteria with few possibilities to develop drug resistance. However, the photosensitizers (PSs) are not effective against Gram-negative bacteria in many cases. Herein, we use conjugated meso-tetra(4-carboxyphenyl)porphine (TCPP) and triaminoguanidinium chloride (TG) to construct self-assembled cationic TCPP-TG nanoparticles (NPs) for efficient bacterial inactivation under visible light illumination. The TCPP-TG NPs can rapidly adhere to both Gram-negative and Gram-positive bacteria and display promoted singlet oxygen (O-1(2)) generation compared with TCPP under light irradiation. The high local positive charge density of TCPP-TG NPs facilitates the interaction between the NPs and bacteria. Consequently, the TCPP-TG NPs produce an elevated concentration of local O-1(2) under light irradiation, resulting in an extraordinarily high antibacterial efficiency (99.9999% inactivation of the representative bacteria within 4 min). Furthermore, the TCPP-TG NPs show excellent water dispersity and stability during 4 months of storage. Therefore, the rationally designed TCPP-TG NPs are a promising antibacterial agent for effective aPDT.
引用
收藏
页码:54378 / 54386
页数:9
相关论文
共 65 条
[1]   An insight on bacterial cellular targets of photodynamic inactivation [J].
Alves, Eliana ;
Faustino, Maria A. F. ;
Neves, Maria G. P. M. S. ;
Cunha, Angela ;
Tome, Joao ;
Almeida, Adelaide .
FUTURE MEDICINAL CHEMISTRY, 2014, 6 (02) :141-164
[2]   Charge effect on the photoinactivation of Gram-negative and Gram-positive bacteria by cationic meso-substituted porphyrins [J].
Alves, Eliana ;
Costa, Liliana ;
Carvalho, Carla M. B. ;
Tome, Joao P. C. ;
Faustino, Maria A. ;
Neves, Maria G. P. M. S. ;
Tome, Augusto C. ;
Cavaleiro, Jose A. S. ;
Cunha, Angela ;
Almeida, Adelaide .
BMC MICROBIOLOGY, 2009, 9
[3]   A comparative in vitro evaluation of cytotoxic effects of EDTA and maleic acid: Root canal irrigants [J].
Ballal, N. Vasudev ;
Kundabala, M. ;
Bhat, Seetharama ;
Rao, Nageshwar ;
Rao, B. S. Satish .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2009, 108 (04) :633-638
[4]   Type I and Type II Photosensitized Oxidation Reactions: Guidelines and Mechanistic Pathways [J].
Baptista, Mauricio S. ;
Cadet, Jean ;
Di Mascio, Paolo ;
Ghogare, Ashwini A. ;
Greer, Alexander ;
Hamblin, Michael R. ;
Lorente, Carolina ;
Nunez, Silvia Cristina ;
Ribeiro, Martha Simoes ;
Thomas, Andres H. ;
Vignoni, Mariana ;
Yoshimura, Tania Mateus .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2017, 93 (04) :912-919
[5]  
BERTOLONI G, 1990, FEMS MICROBIOL LETT, V71, P149
[6]   Synthesis of Guanidinium Functionalized Polycarbodiimides and Their Antibacterial Activities [J].
Budhathoki-Uprety, Januka ;
Peng, LingLing ;
Melander, Christian ;
Novak, Bruce M. .
ACS MACRO LETTERS, 2012, 1 (03) :370-374
[7]   Polyhedral Oligomeric Silsesquioxane (POSS)-Based Cationic Conjugated Oligoelectrolyte/Porphyrin for Efficient Energy Transfer and Multiamplified Antimicrobial Activity [J].
Chen, Jia ;
Shan, Jingyang ;
Xu, Yu ;
Su, Peng ;
Tong, Li ;
Yuwen, Lihui ;
Weng, Lixing ;
Bao, Biqing ;
Wang, Lianhui .
ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (40) :34455-34463
[8]   Supramolecular Porphyrin Photosensitizers: Controllable Disguise and Photoinduced Activation of. Antibacterial Behavior [J].
Chen, Linghui ;
Bai, Haotian ;
Xu, Jiang-Fei ;
Wang, Shu ;
Zhang, Xi .
ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (16) :13950-13957
[9]   One-Step Synthesis of Epoxy Group-Terminated Organosilica Nanodots: A Versatile Nanoplatform for Imaging and Eliminating Multidrug-Resistant Bacteria and Their Biofilms [J].
Chen, Xiaokai ;
Zhang, Xiaodong ;
Lin, Fengming ;
Guo, Yuxin ;
Wu, Fu-Gen .
SMALL, 2019, 15 (37)
[10]   Enzyme Mimicry for Combating Bacteria and Biofilms [J].
Chen, Zhaowei ;
Wang, Zhenzhen ;
Ren, Jinsong ;
Qu, Xiaogang .
ACCOUNTS OF CHEMICAL RESEARCH, 2018, 51 (03) :789-799