Anti-inflammatory cembranolides from the soft coral Lobophytum durum

被引：40

作者：

Cheng, Shi-Yie ^{[1
]}

Wen, Zhi-Hong ^{[1
]}

Wang, Shang-Kwei ^{[2
]}

Chiou, Shu-Fen ^{[1
]}

Hsu, Chi-Hsin ^{[1
,4
]}

Dai, Chang-Feng ^{[3
]}

Duh, Chang-Yih ^{[1
,4
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 804, Taiwan

[2] Kaohsiung Med Univ, Dept Microbiol, Kaohsiung 807, Taiwan

[3] Natl Taiwan Univ, Inst Oceanog, Taipei 10764, Taiwan

[4] Natl Sun Yat Sen Univ, Ctr Asia Pacific Marine Res, Kaohsiung 804, Taiwan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2009年 / 17卷 / 11期

关键词：

Lobophytum durum; Cembranolides; Antibacterial activities; Anti-inflammatory effects; HCMV endonuclease activity;

D O I：

10.1016/j.bmc.2009.04.053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical investigation of the soft coral Lobophytum durum resulted in the isolation of seven new cembranolides, durumolides F-L (1-7), as well as one previously characterized cembranolides, sinularolide D (8). The molecular structures of these isolated metabolites were determined mainly through NMR techniques and HRESIMS analysis. Moreover, the absolute configurations of 1 and 5 were established by application of modified Mosher's method. The antibacterial activities, anti-inflammatory effects, and anti-HCMV (Human cytomegalovirus) endonuclease activity of metabolites 1-8 were also evaluated in vitro. Anti-inflammatory activity of metabolites 1 and 6 (10 mu M) significantly reduced the levels of the iNOS protein to 0.8 +/- 0.6% and 5.7 +/- 2.2%, respectively, and COX-2 protein to 47.8 +/- 9.0% and 71.6 +/- 5.8%, respectively. Metabolites 1-8 (100 mu g/disk) exhibited weak antibacterial activity against Salmonella enteritidis. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：3763 / 3769

页数：7

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