Transcriptomic response to elevated water temperatures in adult migrating Yukon River Chinook salmon (Oncorhynchus tshawytscha)

被引：19

作者：

Bowen, Lizabeth ^{[1
]}

von Biela, Vanessa R. ^{[2
]}

McCormick, Stephen D. ^{[3
,4
]}

Regish, Amy M. ^{[3
]}

Waters, Shannon C. ^{[1
]}

Durbin-Johnson, Blythe ^{[5
]}

Britton, Monica ^{[5
]}

Settles, Matthew L. ^{[5
]}

Donnelly, Daniel S. ^{[2
,7
]}

Laske, Sarah M. ^{[2
]}

Carey, Michael P. ^{[2
]}

Brown, Randy J. ^{[6
]}

Zimmerman, Christian E. ^{[2
]}

机构：

[1] US Geol Survey, Western Ecol Res Ctr, One Shields Ave, Davis, CA 95616 USA

[2] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA

[3] US Geol Survey, Leetown Sci Ctr, Conte Anadromous Fish Res Lab, 1 Migratory Way, Turners Falls, MA 01376 USA

[4] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA

[5] Univ Calif Davis, Genome Ctr & Bioinformat Core Facil, One Shields Ave, Davis, CA 95616 USA

[6] US Fish & Wildlife Serv, 101 12th Ave,Room 110, Fairbanks, AK 99701 USA

[7] US Forest Serv, Chugach Natl Forest, POB 208, Cordova, AK 99574 USA

来源：

CONSERVATION PHYSIOLOGY | 2020年 / 8卷

关键词：

Chinook salmon; transcriptome analysis; heat stress; Yukon River;

D O I：

10.1093/conphys/coaa084

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Chinook salmon (Oncorhynchus tshawytscha) declines are widespread and may be attributed, at least in part, to warming river temperatures. Water temperatures in the Yukon River and tributaries often exceed 18 degrees C, a threshold commonly associated with heat stress and elevated mortality in Pacific salmon. Untangling the complex web of direct and indirect physiological effects of heat stress on salmon is difficult in a natural setting with innumerable system challenges but is necessary to increase our understanding of both lethal and sublethal impacts of heat stress on populations. The goal of this study was to characterize the cellular stress response in multiple Chinook salmon tissues after acute elevated temperature challenges. We conducted a controlled 4-hour temperature exposure (control, 18 degrees C and 21 degrees C) experiment on the bank of the Yukon River followed by gene expression (GE) profiling using a 3'-Tag-RNA-Seq protocol. The full transcriptome was analysed for 22 Chinook salmon in muscle, gill and liver tissue. Both the 21 degrees C and 18 degrees C treatments induced greater activity in genes associated with protein folding (e.g. HSP70, HSP90 mRNA) processes in all tissues. Global GE patterns indicate that transcriptomic responses to heat stress were highly tissue-specific, underscoring the importance of analyzing multiple tissues for determination of physiological effect. Primary superclusters (i.e. groupings of loosely related terms) of altered biological processes were identified in each tissue type, including regulation of DNA damage response (gill), regulation by host of viral transcription (liver) and regulation of the force of heart contraction (muscle) in the 21 degrees C treatment. This study provides insight into mechanisms potentially affecting adult Chinook salmon as they encounter warm water during their spawning migration in the Yukon River and suggests that both basic and more specialized cellular functions may be disrupted.

引用

页数：7

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