Nitrogen deficit decreases seed Cry1Ac endotoxin expression in Bt transgenic cotton

被引：19

作者：

Chen, Yuan ^{[1
]}

Li, Yabing ^{[2
]}

Zhou, Mingyuan ^{[1
]}

Cai, Zezhou ^{[1
]}

Tambel, Leila I. M. ^{[3
]}

Zhang, Xiang ^{[1
]}

Chen, Yuan ^{[1
]}

Chen, Dehua ^{[1
]}

机构：

[1] Yangzhou Univ, Agr Coll, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Jiangsu Key Lab Crops Genet & Physiol,Jiangsu Key, Yangzhou 225009, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang 455000, Henan, Peoples R China

[3] Biotechnol & Biosafety Res Ctr, Agr Res Cooperat, Khartoum, Sudan

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2019年 / 141卷

基金：

中国国家自然科学基金;

关键词：

Bt cotton; Nitrogen deficit; Cry1Ac endotoxin; Boll development; Nitrogen metabolism; INSECTICIDAL PROTEIN-CONTENT; TEMPERATURE; LEPIDOPTERA; IMPACT; SIZE;

D O I：

10.1016/j.plaphy.2019.05.017

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The spatial and temporal expression of insecticidal gene Cry1Ac in transgenic Bacillus thuringiensis (Bt) cotton varies with plant organs, plant age, as well as environmental conditions. The research was undertaken to study the impact of nitrogen deficit on Cry1Ac endotoxin concentration in seed in Yangtze River valley region in China in 2015 and 2016. To uncover the underlying mechanism, the nitrogen metabolism process including protein synthesis and degradation was characterized. Based on the results, nitrogen deficit decreased the boll weight, boll volume, boll number per plant, seed Cry1Ac endotoxin concentration, glutamate oxaloacetate transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities, soluble protein concentration, but increased peptidase and protease activities, and free amino acid content in seed. Our results suggested that the decline of seed Cry1Ac endotoxin expression associated with decreased nitrogen was due to the enhanced protein decomposition and reduced protein synthesis, especially the altered activities of GOT and peptidase. There was a significant negative correlation between seed Cry1Ac endotoxin concentration and boll shell Cry1Ac endotoxin content under nitrogen deficiency. Therefore, seed Cry1Ac endotoxin concentration and boll shell Cry1Ac endotoxin concentration should be balanced to guarantee the insecticidal efficiency.

引用

页码：114 / 121

页数：8

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