Induction of lipocalin-type prostaglandin D synthase in mouse heart under hypoxemia

被引：26

作者：

Han, Feng ^{[2
]}

Takeda, Kazuhisa

Ishikawa, Kazunobu ^{[3
]}

Ono, Masao ^{[4
]}

Date, Fumiko ^{[4
]}

Yokoyama, Satoru

Furuyama, Kazumichi

Shinozawa, Yotaro ^{[2
]}

Urade, Yoshihiro ^{[5
]}

Shibahara, Shigeki ^{[1
]}

机构：

[1] Tohoku Univ, Dept Mol Biol & Appl Physiol, Sch Med, Aoba Ku, Sendai, Miyagi 9808575, Japan

[2] Tohoku Univ, Dept Emergency & Crit Care Med, Sch Med, Sendai, Miyagi 9808575, Japan

[3] Fukushima Med Univ, Dept Internal Med 1, Fukushima, Japan

[4] Tohoku Univ, Div Histopathol, Grad Sch Med, Sendai, Miyagi 9808575, Japan

[5] Osaka Biosci Inst, Dept Mol Behav Biol, Osaka, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 385卷 / 03期

关键词：

Prostaglandin D synthase; Heme oxygenase; Hypoxia; Prostaglandin D-2; Lung; Heart; Pulmonary venous myocardium; MESSENGER-RNA EXPRESSION; PIGMENT EPITHELIAL-CELLS; BETA-TRACE; HEME OXYGENASE-1; PULMONARY-HYPERTENSION; CORONARY-CIRCULATION; SEPARATE REGULATION; ENDOTHELIAL-CELLS; HUMAN BRAIN; HYPOXIA;

D O I：

10.1016/j.bbrc.2009.05.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypoxemia is a common manifestation of various disorders and generates pressure overload to the heart. Here we analyzed the expression of lipocalin-type prostaglandin D synthase (L-PGDS) in the heart of C57BL/6 mice kept Under normobaric hypoxia (10% O-2) that generates hemodynamic stress. Northern and Western blot analyses revealed that the expression levels of L-PGDS mRNA and protein wet e significantly increased (>twofold) after 14 days of hypoxia, compared to the mice kept under normoxia. immunohistochemical analysis indicated that L-PGDS was increased in the myocardium of auricles and ventricles and the Pulmonary venous myocardium at 28 days of hypoxia. Moreover, Using C57BL/6 mice lacking heme oxygenase-2 (HO-2(-/-)) a model of chronic hypoxemia, we showed that the expression level of L-PGDS protein was twofold higher in the heart than that of wild-type mouse. L-PGDS expression is induced in the myocardium Under hypoxemia, which may reflect the adaptation to the hemodynamic stress, (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：449 / 453

页数：5

共 33 条

[1] Hypoxemia and blunted hypoxic ventilatory responses in mice lacking heme oxygenase-2 [J].

Adachi, T ;

Ishikawa, K ;

Hida, W ;

Matsumoto, H ;

Masuda, T ;

Date, F ;

Ogawa, K ;

Takeda, K ;

Furuyama, K ;

Zhang, YZ ;

Kitamuro, T ;

Ogawa, H ;

Maruyama, Y ;

Shibahara, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 320 (02) :514-522

[2] Prostaglandin D synthase (β trace) levels in sleep apnea patients with and without sleepiness [J].

Barcelo, A. ;

de la Pena, M. ;

Barbe, F. ;

Pierola, J. ;

Bosch, M. ;

Agusti, A. G. N. .

SLEEP MEDICINE, 2007, 8 (05) :509-511

[3]

Beuckmann CT, 1996, J NEUROSCI, V16, P6119

[4] Binding of biliverdin, bilirubin, and thyroid hormones to lipocalin-type prostaglandin D synthase [J].

Beuckmann, CT ;

Aoyagi, M ;

Okazaki, I ;

Hiroike, T ;

Toh, H ;

Hayaishi, O ;

Urade, Y .

BIOCHEMISTRY, 1999, 38 (25) :8006-8013

[5] Choroid plexus: The major site of mRNA expression for the beta-trace protein (prostaglandin D synthase) in human brain [J].

Blodorn, B ;

Mader, M ;

Urade, Y ;

Hayaishi, O ;

Felgenhauer, K ;

Bruck, W .

NEUROSCIENCE LETTERS, 1996, 209 (02) :117-120

[6] Hypoxic pulmonary vasoconstriction [J].

Dumas, JP ;

Bardou, M ;

Goirand, F ;

Dumas, M .

GENERAL PHARMACOLOGY, 1999, 33 (04) :289-297

[7] Expression of lipocalin-type prostaglandin D synthase (β-trace) in human heart and its accumulation in the coronary circulation of angina patients [J].

Eguchi, Y ;

Eguchi, N ;

Oda, H ;

Seiki, K ;

Kijima, Y ;

Matsu-ura, Y ;

Urade, Y ;

Hayaishi, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14689-14694

[8] Heme as a magnificent molecule with multiple missions: Heme determines its own fate and governs cellular homeostasis [J].

Furuyama, Kazumichi ;

Kaneko, Kiriko ;

Vargas V., Patrick D. .

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2007, 213 (01) :1-16

[9] Identification of a fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase [J].

Gerena, RL ;

Irikura, D ;

Urade, Y ;

Eguchi, N ;

Chapman, DA ;

Killian, GJ .

BIOLOGY OF REPRODUCTION, 1998, 58 (03) :826-833

[10] Dynamic changes in expression of heme oxygenases in mouse heart and liver during hypoxia [J].

Han, F ;

Takeda, K ;

Yokoyama, S ;

Ueda, H ;

Shinozawa, Y ;

Furuyama, K ;

Shibahara, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 338 (01) :653-659

← 1 2 3 4 →