Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote

被引：91

作者：

Ueda, Minako ^{[1
,2
,3
,4
]}

Aichinger, Ernst ^{[1
]}

Gong, Wen ^{[1
]}

Groot, Edwin ^{[1
]}

Verstraeten, Inge ^{[5
,6
]}

Lam Dai Vu ^{[5
,6
,7
,8
]}

De Smet, Ive ^{[5
,6
]}

Higashiyama, Tetsuya ^{[2
,3
,9
]}

Umeda, Masaaki ^{[4
,10
]}

Laux, Thomas ^{[1
]}

机构：

[1] Albert Ludwigs Univ Freiburg, Fac Biol, BIOSS Ctr Biol Signaling Studies, D-79104 Freiburg, Germany

[2] Nagoya Univ, ITbM, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648601, Japan

[3] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648602, Japan

[4] Nara Inst Sci & Technol NAIST, Grad Sch Biol Sci, Lab Plant Growth Regulat, Nara 6300192, Japan

[5] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[6] VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium

[7] VIB, Med Biotechnol Ctr, B-9000 Ghent, Belgium

[8] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

[9] Nagoya Univ, Higashiyama Live Holon Project, Japan Sci & Tech Agcy JST, Exploratory Res Adv Technol ERATO,Chikusa Ku, Furo Cho, Nagoya, Aichi 4648602, Japan

[10] JST, CREST, Nara 6300192, Japan

来源：

GENES & DEVELOPMENT | 2017年 / 31卷 / 06期

基金：

日本学术振兴会;

关键词：

zygote asymmetry; MAPK signaling; WOX8; maternal factors; APICAL-BASAL AXIS; EMBRYO DEVELOPMENT; EARLY EMBRYOGENESIS; GENE-EXPRESSION; DNA METHYLATION; CELL FATE; THALIANA; DYNAMICS; ENDOSPERM; PROTEINS;

D O I：

10.1101/gad.292409.116

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. Unlike animals, plant zygotes are transcriptionally active, implying that plants have evolved specific mechanisms to control transcriptional activation of patterning genes in the zygote. In Arabidopsis, two pathways have been found to regulate zygote asymmetry: YODA (YDA) mitogen-activated protein kinase (MAPK) signaling, which is potentiated by sperm-delivered mRNA of the SHORT SUSPENSOR (SSP) membrane protein, and up-regulation of the patterning gene WOX8 by the WRKY2 transcription factor. How SSP/YDA signaling is transduced into the nucleus and how these pathways are integrated have remained elusive. Here we show that paternal SSP/YDA signaling directly phosphorylates WRKY2, which in turn leads to the up-regulation of WOX8 transcription in the zygote. We further discovered the transcription factors HOMEODOMAIN GLABROUS11/12 (HDG11/12) as maternal regulators of zygote asymmetry that also directly regulate WOX8 transcription. Our results reveal a framework of how maternal and paternal factors are integrated in the zygote to regulate embryo patterning.

引用

页码：617 / 627

页数：11

共 56 条

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