A sensitive immunoassay based on fluorescence resonance energy transfer from up-converting nanoparticles and graphene oxide for one-step detection of imidacloprid

被引:54
作者
Guo, Yirong [1 ]
Zou, Rubing [1 ]
Si, Fangfang [1 ]
Liang, Wenlong [1 ]
Zhang, Tianyi [1 ]
Chang, Yunyun [1 ]
Qiao, Xvsheng [2 ,3 ]
Zhao, Jinhao [1 ]
机构
[1] Zhejiang Univ, Inst Pesticide & Environm Toxicol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China
[2] Zhejiang Univ, State Key Lab Silicon Mat, Hangzhou 310027, Peoples R China
[3] Zhejiang Univ, Sch Mat Sci & Engn, Hangzhou 310027, Peoples R China
基金
中国国家自然科学基金;
关键词
Imidacloprid; Fluorescence resonance energy transfer; Graphene oxide; Up-converting nanoparticles; Immunoassay; LINKED-IMMUNOSORBENT-ASSAY; NEONICOTINOID INSECTICIDES; CONVERSION NANOPARTICLES; LIQUID-CHROMATOGRAPHY; QUANTUM DOTS; HONEY-BEE; METABOLITES; FRET; BIOSENSOR; EXPOSURE;
D O I
10.1016/j.foodchem.2020.127609
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this study, a fluorescence resonance energy transfer (FRET) immunoassay based on graphene oxide (GO) and up-converting nanoparticles (UCNPs) was established for rapid detection of imidacloprid, a commonly-used insecticide. Under 980 nm near-infrared light excitation, emission of UCNPs at 542 nm can be absorbed by the energy acceptor GO. The carboxyl-functionalized GO and UCNPs were coupled with competitive antigen and antibody against imidacloprid. After optimization, the FRET immunoassay showed a wide detection range of 0.08-50 ng/mL to imidacloprid, with cross-reaction toward other three neonicotinoids including imidaclothiz (74.4%), thiacloprid (36.9%) and clothianidin (31.9%). The average recoveries of spiked water, Chinese cabbage, cucumber, honey and tea samples were 76.8%-101.8%. The accuracy and reliability of the FRET immunoassay were verified by UPLC-MS/MS with a good correlation (R-2 = 0.9816). In a summary, this study provides a sensitive and one-step method for monitoring imidacloprid residue in food and environmental samples within 1 h.
引用
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页数:8
相关论文
共 35 条
[1]   FRET as a biomolecular research tool-understanding its potential while avoiding pitfalls [J].
Algar, W. Russ ;
Hildebrandt, Niko ;
Vogel, Steven S. ;
Medintz, Igor L. .
NATURE METHODS, 2019, 16 (09) :815-829
[2]   Highly Sensitive DNA Sensor Based on Upconversion Nanoparticles and Graphene Oxide [J].
Alonso-Cristobal, P. ;
Vilela, P. ;
El-Sagheer, A. ;
Lopez-Cabarcos, E. ;
Brown, T. ;
Muskens, O. L. ;
Rubio-Retama, J. ;
Kanaras, A. G. .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (23) :12422-12429
[3]   An efficient fluorescence resonance energy transfer system from quantum dots to graphene oxide nano sheets: Application in a photoluminescence aptasensing probe for the sensitive detection of diazinon [J].
Arvand, Majid ;
Mirroshandel, Aazam A. .
FOOD CHEMISTRY, 2019, 280 :115-122
[4]   A Graphene Oxide-Organic Dye Ionic Complex with DNA-Sensing and Optical-Limiting Properties [J].
Balapanuru, Janardhan ;
Yang, Jia-Xiang ;
Xiao, Si ;
Bao, Qiaoliang ;
Jahan, Maryam ;
Polavarapu, Lakshminarayana ;
Wei, Ji ;
Xu, Qing-Hua ;
Loh, Kian Ping .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (37) :6549-6553
[5]   Determination of neonicotinoid insecticides and their metabolites in honey bee and honey by liquid chromatography tandem mass spectrometry [J].
Gbylik-Sikorska, Malgorzata ;
Sniegocki, Tomasz ;
Posyniak, Andrzej .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2015, 990 :132-140
[6]   QUANTIFICATION OF IMIDACLOPRID IN HONEYBEES: DEVELOPMENT OF A CHEMILUMINESCENT ELISA [J].
Girotti, Stefano ;
Maiolini, Elisabetta ;
Ghini, Severino ;
Eremin, Sergei A. ;
Manes, Jordi .
ANALYTICAL LETTERS, 2010, 43 (03) :466-475
[7]   Human exposure to neonicotinoid insecticides and the evaluation of their potential toxicity: An overview [J].
Han, Wenchao ;
Tian, Ying ;
Shen, Xiaoming .
CHEMOSPHERE, 2018, 192 :59-65
[8]   Monoclonal antibody-based enzyme-linked immunosorbent assay for the insecticide imidacloprid [J].
Kim, HJ ;
Shelver, WL ;
Li, QX .
ANALYTICA CHIMICA ACTA, 2004, 509 (01) :111-118
[9]   Development of a simple extraction and oxidation procedure for the residue analysis of imidacloprid and its metabolites in lettuce using gas chromatography [J].
Ko, Ah-Young ;
Rahman, Md. Musfiqur ;
Abd El-Aty, A. M. ;
Jang, Jin ;
Park, Jong-Hyouk ;
Cho, Soon-Kil ;
Shim, Jae-Han .
FOOD CHEMISTRY, 2014, 148 :402-409
[10]  
Larson DR, 2003, SCIENCE, V300, P1434, DOI 10.1126/science.1083780